Immunology Antibodies 1

NF-kB p65/RelA Rabbit Polyclonal Antibody (CAB11204)

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SKU:
CAB11204
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology
Reactivity:
Human, Mouse, Rat
Synonyms:
p65, CMCU, NFKB3, AIF3BL3, NF-kB p65/RelA
Research Use:
Immunology
Frequently bought together:

Description

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NF-kB p65/RelA Antibody (CAB11204)

The NF-kB p65/RelA Antibody (CAB11204) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it ideal for Western blot applications.RELA, also known as p65, is a crucial transcription factor that regulates the expression of numerous genes involved in immune response, cell survival, and inflammatory processes.

This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:NF-kB p65/RelA Antibody
SKU:CAB11204
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:LGAL LGNS TDPA VFTD LASV DNSE FQQL LNQG IPVA PHTT EPML MEYP EAIT RLVT GAQR PPDP APAP LGAP GLPN GLLS GDED FSSI ADMD FSAL LSQI SS
Tested Applications:WB IF/ICC IP ELISA
Recommended Dilution:
WB1:500 - 1:2000
IF/ICC1:50 - 1:200
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:p65, CMCU, NFKB3, AIF3BL3, NF-kB p65/RelA
Positive Sample:293T
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:60kDa
Observed MW:65kDa

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.

Purification MethodAffinity purification
Gene ID5970
RRIDAB_2758458
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from 293T cells, using NF-kB p65/RelA Rabbit pAb (CAB11204) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Product ImageImmunofluorescence analysis of U2OS cells using NF-kB p65/RelA Rabbit pAb (CAB11204) at dilution of 1:100. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of PC-12 cells using NF-kB p65/RelA Rabbit pAb (CAB11204) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of U-2 OS cells using NF-kB p65/RelA Rabbit pAb (CAB11204) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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