The Neutrophil Elastase Monoclonal Antibody (CAB8953) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, generated in rabbits, exhibits high specificity towards human samples and has been validated for use in Western blot applications. By targeting the ELANE protein, this antibody enables the detection and analysis of neutrophil elastase in different cell types, making it ideal for investigations in immunology and inflammation research.Neutrophil elastase, also known as ELANE, plays a crucial role in the innate immune response by breaking down proteins and regulating inflammatory pathways.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Neutrophil Elastase Monoclonal Antibody
SKU:
CAB8953
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC1364
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. The enzyme may play a role in degenerative and inflammatory diseases through proteolysis of collagen-IV and elastin. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19.
Purification Method
Affinity purification
Gene ID
1991
RRID
AB_3717878
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Neutrophil Elastase (ELANE) Rabbit mAb (CAB8953) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC): Jurkat Exposure time: 5s.
Immunoprecipitation of Neutrophil Elastase (ELANE) from 300 µg extracts of THP-1 cells was performed using 3 µg of Neutrophil Elastase (ELANE) Rabbit mAb (CAB8953). Rabbit Control IgG (AC005) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using Neutrophil Elastase (ELANE) Rabbit mAb (CAB8953) at a dilution of 1:500.
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