Cell Death Antibodies 2

Anti-NEK6 Antibody (CAB8481)

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SKU:
CAB8481
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Death

Description

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Antibody Name:Anti-NEK6 Antibody
Antibody SKU:CAB8481
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 35-347 of human NEK6 (NP_001159643.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 35-347 of human NEK6 (NP_001159643.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAGQ PGHM PHGG SSNN LCHT LGPV HPPD PQRH PNTL SFRC SLAD FQIE KKIG RGQF SEVY KATC LLDR KTVA LKKV QIFE MMDA KARQ DCVK EIGL LKQL NHPN IIKY LDSF IEDN ELNI VLEL ADAG DLSQ MIKY FKKQ KRLI PERT VWKY FVQL CSAV EHMH SRRV MHRD IKPA NVFI TATG VVKL GDLG LGRF FSSE TTAA HSLV GTPY YMSP ERIH ENGY NFKS DIWS LGCL LYEM AALQ SPFY GDKM NLFS LCQK IEQC DYPP LPGE HYSE KLRE LVSM CICP DPHQ RPDI GYVH QVAK QMHI WMSS T
Gene ID:10783
Uniprot:Q9HC98
Cellular Location:Cytoplasm, Nucleus, Nucleus speckle, centrosome, cytoskeleton, microtubule organizing center, spindle pole
Calculated MW:35kDa/37kDa/38kDa/39kDa
Observed MW:36kDa
Synonyms:NEK6, SID6-1512
Background:The protein encoded by this gene is a kinase required for progression through the metaphase portion of mitosis. Inhibition of the encoded protein can lead to apoptosis. This protein also can enhance tumorigenesis by suppressing tumor cell senescence. Several transcript variants encoding a few different isoforms have been found for this gene.
UniProt Protein Function:NEK6: a protein kinase of the NEK family. Important in mitosis. May regulate spindle function.
UniProt Protein Details:

Protein type:Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Protein kinase, Other; Other group; NEK family

Chromosomal Location of Human Ortholog: 9q33.3-q34.11

Cellular Component: cytoplasm; cytosol; intracellular membrane-bound organelle; microtubule organizing center; nucleoplasm; nucleus

Molecular Function:ATP binding; kinesin binding; magnesium ion binding; protein binding; protein kinase binding; protein serine/threonine kinase activity; signal transducer activity

Biological Process: chromosome segregation; cytokinesis; G2/M transition DNA damage checkpoint; mitotic nuclear envelope disassembly; peptidyl-serine phosphorylation; positive regulation of I-kappaB kinase/NF-kappaB cascade; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of mitotic cell cycle; regulation of mitotic metaphase/anaphase transition; spindle assembly

NCBI Summary:The protein encoded by this gene is a kinase required for progression through the metaphase portion of mitosis. Inhibition of the encoded protein can lead to apoptosis. This protein also can enhance tumorigenesis by suppressing tumor cell senescence. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
UniProt Code:Q9HC98
NCBI GenInfo Identifier:37537993
NCBI Gene ID:10783
NCBI Accession:Q9HC98.2
UniProt Secondary Accession:Q9HC98,Q5TBG3, Q5TBG9, Q6FG86, Q6IAR3, Q96E83, Q9ULX2 B7Z2D9,
UniProt Related Accession:Q9HC98
Molecular Weight:38,460 Da
NCBI Full Name:Serine/threonine-protein kinase Nek6
NCBI Synonym Full Names:NIMA related kinase 6
NCBI Official Symbol:NEK6  
NCBI Official Synonym Symbols:SID6-1512  
NCBI Protein Information:serine/threonine-protein kinase Nek6
UniProt Protein Name:Serine/threonine-protein kinase Nek6
UniProt Synonym Protein Names:Never in mitosis A-related kinase 6; NimA-related protein kinase 6; Protein kinase SID6-1512
Protein Family:Serine/threonine-protein kinase
UniProt Gene Name:NEK6  
UniProt Entry Name:NEK6_HUMAN
Anti-NEK6 Polyclonal Antibody (CAB8481)Western blot analysis of extracts of 293T cells, using NEK6 Antibody (CAB8481) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
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