Name: Anti-NEIL-1 Antibody (CAB5828)
Brand: Epigenetics & Nuclear Signaling Antibodies 2
SKU: CAB5828
Availability: OutOfStock

Antibody Name:	NEIL1 Rabbit Polyclonal Antibody
Antibody SKU:	CAB5828
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 187-476 of human NEIL1 (NP_001243481.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, MCF7, Mouse spleen, Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 187-476 of human NEIL1 (NP_001243481.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FYTA PPGP RLAL CFVD IRRF GRWD LGGK WQPG RGPC VLQE YQQF RENV LRNL ADKA FDRP ICEA LLDQ RFFN GIGN YLRA EILY RLKI PPFE KARS VLEA LQQH RPSP ELTL SQKI RTKL QNPD LLEL CHSV PKEV VQLG GKGY GSES GEED FAAF RAWL RCYG MPGM SSLQ DRHG RTIW FQGD PGPL APKG RKSR KKKS KATQ LSPE DRVE DALP PSKA PSRT RRAK RDLP KRTA TQRP EGTS LQQD PEAP TVPK KGRR KGRQ AASG HCRP RKVK ADIP SLEP EGTS AS
Gene ID:	79661
Uniprot:	Q96FI4
Cellular Location:	Chromosome, Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center
Calculated MW:	43kDa
Observed MW:	44KDa

Synonyms:	NEIL1, FPG1, NEI1, hFPG1
Background:	This gene is a member of the Nei endonuclease VIII-like gene family which encodes DNA glycosylases. The encoded enzyme participates in the DNA repair pathway by initiating base excision repair by removing damaged bases, primarily oxidized pyrimidines. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	NEIL1: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8- oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. Belongs to the FPG family.
UniProt Protein Details:	Protein type:DNA repair, damage; Hydrolase; EC 4.2.99.18; Lyase Chromosomal Location of Human Ortholog: 15q24.2 Cellular Component: cytoplasm; nucleoplasm; nucleus Molecular Function:DNA N-glycosylase activity; DNA-(apurinic or apyrimidinic site) lyase activity; hydrolase activity, acting on glycosyl bonds; protein C-terminus binding Biological Process: base-excision repair; depyrimidination; negative regulation of nuclease activity; response to oxidative stress
NCBI Summary:	This gene is a member of the Nei endonuclease VIII-like gene family which encodes DNA glycosylases. The encoded enzyme participates in the DNA repair pathway by initiating base excision repair by removing damaged bases, primarily oxidized pyrimidines. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]
UniProt Code:	Q96FI4
NCBI GenInfo Identifier:	56404654
NCBI Gene ID:	79661
NCBI Accession:	Q96FI4.3
UniProt Secondary Accession:	Q96FI4,Q6ZRA7, Q86XW7, Q9H6C3, D3DW75,
UniProt Related Accession:	Q96FI4
Molecular Weight:	43,684 Da
NCBI Full Name:	Endonuclease 8-like 1
NCBI Synonym Full Names:	nei like DNA glycosylase 1
NCBI Official Symbol:	NEIL1
NCBI Official Synonym Symbols:	FPG1; NEI1; hFPG1
NCBI Protein Information:	endonuclease 8-like 1
UniProt Protein Name:	Endonuclease 8-like 1
UniProt Synonym Protein Names:	DNA glycosylase/AP lyase Neil1; DNA-(apurinic or apyrimidinic site) lyase Neil1; Endonuclease VIII-like 1; FPG1; Nei homolog 1; NEH1; Nei-like protein 1
Protein Family:	Endonuclease
UniProt Gene Name:	NEIL1
UniProt Entry Name:	NEIL1_HUMAN

	Western blot analysis of extracts of Mouse spleen, using NEIL1 antibody (CAB5828) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Western blot analysis of extracts of various cell lines, using NEIL1 antibody (CAB5828) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of U2OS cells using NEIL1 antibody (CAB5828). Blue: DAPI for nuclear staining.