Anti-NEDD4L Antibody (CAB8085)

Antibody Name:	NEDD4L Rabbit Polyclonal Antibody
Antibody SKU:	CAB8085
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 836-955 of human NEDD4L (NP_056092.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	BT-474, Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 836-955 of human NEDD4L (NP_056092.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GDVD VNDW RQHS IYKN GYCP NHPV IQWF WKAV LLMD AEKR IRLL QFVT GTSR VPMN GFAE LYGS NGPQ LFTI EQWG SPEK LPRA HTCF NRLD LPPY ETFE DLRE KLLM AVEN AQGF EGVD
Gene ID:	23327
Uniprot:	Q96PU5
Cellular Location:	Cytoplasm
Calculated MW:	96-111kDa
Observed MW:	113kDa

Synonyms:

NEDD4L, NEDD4-2, NEDD4.2, PVNH7, RSP5, hNEDD4-2

Background:

This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein mediates the ubiquitination of multiple target substrates and plays a critical role in epithelial sodium transport by regulating the cell surface expression of the epithelial sodium channel, ENaC. Single nucleotide polymorphisms in this gene may be associated with essential hypertension. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

UniProt Protein Function:	NEDD4L: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. Promotes ubiquitination and degradation of SGK1 and TNK2. Interacts with UBE2E3. Interacts with NDFIP1 and NDFIP2; this interaction activates the E3 ubiquitin-protein ligase. Interacts via its WW domains with SCNN1A, SCNN1B, SCNN1G, SCN1A, SCN2A, SCN3A, SCN5A, SCN8A, SCN9A, SCN10A and CLCN5. Interacts with SMAD2, SMAD3, SMAD6 and SMAD7. The phosphorylated form interacts with 14-3-3 proteins. Interacts with Epstein-Barr virus LMP2A. Interacts with TNK2. Interacts with WNK1. Interacts with SGK1. By androgens in prostate, and by albumin in kidney. Ubiquitously expressed, with highest levels in prostate, pancreas and kidney. Activated by NDFIP1- and NDFIP2-binding. 9 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 6.3.2.-; Ubiquitin ligase; Ligase; Ubiquitin conjugating system; EC 6.3.2.19 Chromosomal Location of Human Ortholog: 18q21.31 Cellular Component: cytoplasm; cytosol; intracellular; nucleoplasm; nucleus; plasma membrane Molecular Function:potassium channel inhibitor activity; potassium channel regulator activity; protein binding; sodium channel inhibitor activity; sodium channel regulator activity; ubiquitin-protein ligase activity Biological Process: negative regulation of transcription from RNA polymerase II promoter; proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination; protein ubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process; regulation of membrane potential; response to metal ion; viral infectious cycle
NCBI Summary:	This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein mediates the ubiquitination of multiple target substrates and plays a critical role in epithelial sodium transport by regulating the cell surface expression of the epithelial sodium channel, ENaC. Single nucleotide polymorphisms in this gene may be associated with essential hypertension. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]
UniProt Code:	Q96PU5
NCBI GenInfo Identifier:	73921204
NCBI Gene ID:	23327
NCBI Accession:	Q96PU5.2
UniProt Secondary Accession:	Q96PU5,O43165, Q3LSM7, Q7Z5F1, Q7Z5F2, Q7Z5N3, Q8N5A7 Q8WUU9, Q9BW58, Q9H2W4, Q9NT88,
UniProt Related Accession:	Q96PU5
Molecular Weight:	96,271 Da
NCBI Full Name:	E3 ubiquitin-protein ligase NEDD4-like
NCBI Synonym Full Names:	neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase
NCBI Official Symbol:	NEDD4L
NCBI Official Synonym Symbols:	RSP5; NEDD4-2; NEDD4.2; hNEDD4-2
NCBI Protein Information:	E3 ubiquitin-protein ligase NEDD4-like
UniProt Protein Name:	E3 ubiquitin-protein ligase NEDD4-like
UniProt Synonym Protein Names:	NEDD4.2; Nedd4-2
Protein Family:	E3 ubiquitin-protein ligase
UniProt Gene Name:	NEDD4L
UniProt Entry Name:	NED4L_HUMAN

	Western blot analysis of extracts of various cell lines, using NEDD4L antibody (CAB8085) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat kidney using NEDD4L antibody (CAB8085) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded human prostate using NEDD4L antibody (CAB8085) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of L929 cells using NEDD4L antibody (CAB8085) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

Anti-Phospho-NEDD4L-S448 pAb Antibody (CABP0843)

NEDD4L Antibody (PACO10808)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)