Antibody Name:	Anti-NEDD4 Antibody
Antibody SKU:	CAB0552
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 160-460 of human NEDD4 (NP_940682.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human
Positive Samples:	U-251MG, HeLa, Jurkat, A-549, SKOV3

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 160-460 of human NEDD4 (NP_940682.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GSYS SNGS DFGS CASI TSGG SYTN SVIS DSSS YTFP PSDD TFLG GNLP SDST SNRS VPNR NTTP CEIF SRST STDP FVQD DLEH GLEI MKLP VSRN TKIP LKRY SSLV IFPR SPST TRPT SPTS LCTL LSKG SYQT SHQF IISP SEIA HNED GTSA KGFL STAV NGLR LSKT ICTP GEVR DIRP LHRK GSLQ KKIV LSNN TPRQ TVCE KSSE GYSC VSVH FTQR KAAT LDCE TTNG DCKP EMSE IKLN SDSE YIKL MHRT SACL PSSQ NVDC QINI NGEL ERPH SQMN KNHG ILRR SISL G
Gene ID:	4734
Uniprot:	P46934
Cellular Location:	Cell membrane, Cytoplasm, Peripheral membrane protein
Calculated MW:	104kDa/140kDa/147kDa/149kDa
Observed MW:	120kDa

Synonyms:

NEDD4, NEDD4-1, RPF1

Background:

This gene is the founding member of the NEDD4 family of HECT ubiquitin ligases that function in the ubiquitin proteasome system of protein degradation. The encoded protein contains an N-terminal calcium and phospholipid binding C2 domain followed by multiple tryptophan-rich WW domains and, a C-terminal HECT ubiquitin ligase catalytic domain. It plays critical role in the regulation of a number of membrane receptors, endocytic machinery components and the tumor suppressor PTEN.

UniProt Protein Function:	E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Specifically ubiquitinates 'Lys-63' in target proteins (PubMed:23644597). Involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. Monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes. Ubiquitinates FGFR1, leading to receptor internalization and degradation in lysosomes. Promotes ubiquitination of RAPGEF2. According to PubMed:18562292 the direct link between NEDD4 and PTEN regulation through polyubiquitination described in PubMed:17218260 is questionable. Involved in ubiquitination of ERBB4 intracellular domain E4ICD. Involved in the budding of many viruses. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. Ubiquitinates TNK2 and regulates EGF-induced degradation of EGFR and TNF2. Involved in the ubiquitination of ebola virus VP40 protein and this ubiquitination plays a role in facilitating viral budding. Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1 (PubMed:25631046).
UniProt Code:	P46934
NCBI GenInfo Identifier:	313104311
NCBI Gene ID:	4734
NCBI Accession:	P46934.4
UniProt Secondary Accession:	P46934,A1KY35, A6ND72, A7MD29, B4E2R7, B7ZM59, B7ZM60 B9EGN5, D6RF89,
UniProt Related Accession:	P46934
Molecular Weight:	1319
NCBI Full Name:	E3 ubiquitin-protein ligase NEDD4
NCBI Synonym Full Names:	neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase
NCBI Official Symbol:	NEDD4
NCBI Official Synonym Symbols:	RPF1; NEDD4-1
NCBI Protein Information:	E3 ubiquitin-protein ligase NEDD4; receptor-potentiating factor 1; cell proliferation-inducing gene 53 protein; neural precursor cell expressed developmentally down-regulated protein 4
UniProt Protein Name:	E3 ubiquitin-protein ligase NEDD4
UniProt Synonym Protein Names:	Cell proliferation-inducing gene 53 protein; Neural precursor cell expressed developmentally down-regulated protein 4; NEDD-4
Protein Family:	Nedd4 binding protein
UniProt Gene Name:	NEDD4
UniProt Entry Name:	NEDD4_HUMAN

	Western blot analysis of extracts of various cell lines, using NEDD4 antibody (CAB0552) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human prostate using NEDD4 antibody (CAB0552) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded human gastric using NEDD4 antibody (CAB0552) at dilution of 1:200 (40x lens).