Name: Anti-NECAB3 Antibody (CAB15500)
Brand: Cell Biology Antibodies 6
SKU: CAB15500
Availability: OutOfStock

Antibody Name:	Anti-NECAB3 Antibody
Antibody SKU:	CAB15500
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-362 of human NECAB3 (NP_112508.3).

Application:	WB
Recommended Dilution:	WB 1:200 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	LO2, Mouse liver, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-362 of human NECAB3 (NP_112508.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MACA GLLT VCLL RPPA PQPQ PQTP RHPQ LAPD PGPA GHTL FQDV FRRA DKND DGKL SFEE FQNY FADG VLSL GELQ ELFS GIDG HLTD NLET EKLC DYFS EHLG VYRP VLAA LESL NRAV LAAM DATK LEYE RASK VDQF VTRF LLRE TVSQ LQAL QSSL EGAS DTLE AQAH GWRS DAES VEAQ SRLC GSRR AGRR ALRS VSRS STWS PGSS DTGR SSEA EMQW RLQV NRLQ ELID QLEC KAPR LEPL REED LAKG PDLH ILMA QRQV QVAE EGLQ DFHR ALRC YVDF TGAQ SHCL HVSA QKML DGAS FTLY EFWQ DEAS WRRH QQSP GSKA FQRI LIDH LRAP DTLT TVFF PASW WIMN NN
Gene ID:	63941
Uniprot:	Q96P71
Cellular Location:	Golgi apparatus
Calculated MW:	22kDa/40kDa/44kDa
Observed MW:	50kDa

Synonyms:

NECAB3, APBA2BP, EFCBP3, NIP1, STIP3, SYTIP2, XB51, dJ63M2.4, dJ63M2.5

Background:

The protein encoded by this gene interacts with the amino-terminal domain of the neuron-specific X11-like protein (X11L), inhibits the association of X11L with amyloid precursor protein through a non-competitive mechanism, and abolishes the suppression of beta-amyloid production by X11L. This protein, together with X11L, may play an important role in the regulatory system of amyloid precursor protein metabolism and beta-amyloid generation. The protein is phosphorylated by NIMA-related expressed kinase 2, and localizes to the Golgi apparatus. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	NECAB3: Inhibits the interaction of APBA2 with beta-amyloid precursor protein (APP), and hence allows formation of beta- amyloid. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 20q11.22 Cellular Component: endoplasmic reticulum membrane; cytoplasm; Golgi cis cisterna; nucleus Molecular Function:protein binding; calcium ion binding Biological Process: regulation of amyloid precursor protein biosynthetic process; protein metabolic process; protein secretion
NCBI Summary:	The protein encoded by this gene interacts with the amino-terminal domain of the neuron-specific X11-like protein (X11L), inhibits the association of X11L with amyloid precursor protein through a non-competitive mechanism, and abolishes the suppression of beta-amyloid production by X11L. This protein, together with X11L, may play an important role in the regulatory system of amyloid precursor protein metabolism and beta-amyloid generation. The protein is phosphorylated by NIMA-related expressed kinase 2, and localizes to the Golgi apparatus. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q96P71
NCBI GenInfo Identifier:	149158725
NCBI Gene ID:	63941
NCBI Accession:	NP_112508.3
UniProt Secondary Accession:	Q96P71,Q5JWF5, Q5JWF6, Q5JWF7, Q86VV1, Q9H433, Q9H8G8 Q9HBW7, Q9HCQ9, A8K780, E1P5N2,
UniProt Related Accession:	Q96P71
Molecular Weight:	44,350 Da
NCBI Full Name:	N-terminal EF-hand calcium-binding protein 3 isoform 1
NCBI Synonym Full Names:	N-terminal EF-hand calcium binding protein 3
NCBI Official Symbol:	NECAB3
NCBI Official Synonym Symbols:	NIP1; XB51; STIP3; EFCBP3; SYTIP2; APBA2BP; dJ63M2.4; dJ63M2.5
NCBI Protein Information:	N-terminal EF-hand calcium-binding protein 3; X11L-binding protein 51; Nek2-interacting protein 1; EF-hand calcium binding protein 3; neuronal calcium-binding protein 3; synaptotagmin interacting protein 2; neuronal calcium-binding protein NECAB3; synapto
UniProt Protein Name:	N-terminal EF-hand calcium-binding protein 3
UniProt Synonym Protein Names:	Amyloid beta A4 protein-binding family A member 2-binding protein; Nek2-interacting protein 1; Neuronal calcium-binding protein 3; X11L-binding protein 51
Protein Family:	N-terminal EF-hand calcium-binding protein
UniProt Gene Name:	NECAB3
UniProt Entry Name:	NECA3_HUMAN

Western blot analysis of extracts of various cell lines, using NECAB3 antibody (CAB15500) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.

Antibodies

NECAB3 Antibody (PACO00403)

NECAB3 Antibody (PACO10803)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)