KO Validated Antibodies 1

Anti-NDUFS3 Antibody (CAB8013)[KO Validated]

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SKU:
CAB8013
Product Type:
Antibody
Applications:
WB
Applications:
IHC
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:[KO Validated] NDUFS3 Rabbit Polyclonal Antibody
Antibody SKU:CAB8013
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 37-264 of human NDUFS3 (NP_004542.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:MCF-7, SW620, HepG2, Mouse kidney, Mouse liver, Mouse heart, Rat liver, Rat heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 37-264 of human NDUFS3 (NP_004542.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ESAG ADTR PTVR PRND VAHK QLSA FGEY VAEI LPKY VQQV QVSC FNEL EVCI HPDG VIPV LTFL RDHT NAQF KSLV DLTA VDVP TRQN RFEI VYNL LSLR FNSR IRVK TYTD ELTP IESA VSVF KAAN WYER EIWD MFGV FFAN HPDL RRIL TDYG FEGH PFRK DFPL SGYV ELRY DDEV KRVV AEPV ELAQ EFRK FDLN SPWE AFPV YRQP PESL KLEA GDKK PDAK
Gene ID:4722
Uniprot:O75489
Cellular Location:Mitochondrion inner membrane
Calculated MW:14kDa/30kDa
Observed MW:30kDa
Synonyms:NDUFS3, CI-30
Background:This gene encodes one of the iron-sulfur protein (IP) components of mitochondrial NADH:ubiquinone oxidoreductase (complex I). Mutations in this gene are associated with Leigh syndrome resulting from mitochondrial complex I deficiency.
UniProt Protein Function:NDUFS3: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Belongs to the complex I 30 kDa subunit family.
UniProt Protein Details:

Protein type:EC 1.6.5.3; Oxidoreductase; EC 1.6.99.3; Energy Metabolism - oxidative phosphorylation; Mitochondrial

Chromosomal Location of Human Ortholog: 11p11.11

Cellular Component: mitochondrial matrix; mitochondrial membrane; mitochondrial respiratory chain complex I; mitochondrion; nucleus

Molecular Function:NADH dehydrogenase activity; protein binding

Biological Process: mitochondrial electron transport, NADH to ubiquinone; mitochondrial respiratory chain complex I assembly; negative regulation of cell growth; substantia nigra development

Disease: Leigh Syndrome; Mitochondrial Complex I Deficiency

NCBI Summary:This gene encodes one of the iron-sulfur protein (IP) components of mitochondrial NADH:ubiquinone oxidoreductase (complex I). Mutations in this gene are associated with Leigh syndrome resulting from mitochondrial complex I deficiency.[provided by RefSeq, Apr 2009]
UniProt Code:O75489
NCBI GenInfo Identifier:6166589
NCBI Gene ID:4722
NCBI Accession:O75489.1
UniProt Secondary Accession:O75489,Q9UNQ8, B2R9J1, B4DFM8,
UniProt Related Accession:O75489
Molecular Weight:14,679 Da
NCBI Full Name:NADH dehydrogenase
NCBI Synonym Full Names:NADH:ubiquinone oxidoreductase core subunit S3
NCBI Official Symbol:NDUFS3  
NCBI Official Synonym Symbols:CI-30  
NCBI Protein Information:NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial
UniProt Protein Name:NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial
UniProt Synonym Protein Names:Complex I-30kD; CI-30kD; NADH-ubiquinone oxidoreductase 30 kDa subunit
UniProt Gene Name:NDUFS3  
UniProt Entry Name:NDUS3_HUMAN
Anti-NDUFS3 Polyclonal Antibody [KO Validated] (CAB8013)Western blot analysis of extracts of various cell lines, using NDUFS3 antibody (CAB8013) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-NDUFS3 Polyclonal Antibody [KO Validated] (CAB8013)Western blot analysis of extracts from normal (control) and NDUFS3 knockout (KO) 293T cells, using NDUFS3 antibody (CAB8013) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-NDUFS3 Polyclonal Antibody [KO Validated] (CAB8013)Immunohistochemistry of paraffin-embedded human liver damage using NDUFS3 antibody (CAB8013) at dilution of 1:100 (40x lens).
Anti-NDUFS3 Polyclonal Antibody [KO Validated] (CAB8013)Immunohistochemistry of paraffin-embedded rat brain using NDUFS3 antibody (CAB8013) at dilution of 1:100 (40x lens).
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