Metabolism Antibodies 3
Anti-NDUFB8 Monoclonal Antibody (CAB19732)
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- SKU:
- CAB19732
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Research Area:
- Metabolism
Description
system_update_altMSDS
| Antibody Name: | Anti-NDUFB8 Monoclonal Antibody |
| Antibody SKU: | CAB19732 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthesized peptide derived from human NDUFB8 |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:1000 - 1:3000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, HepG2, RAW264, 7, THP-1, C6, Mouse kidney, Mouse heart, Rat kidney, Rat heart |
| Immunogen: | A synthesized peptide derived from human NDUFB8 |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 4714 |
| Uniprot: | O95169 |
| Cellular Location: | |
| Calculated MW: | 19kDa |
| Observed MW: | 22KDa |
| Synonyms: | ASHI; CI-ASHI |
| UniProt Protein Function: | NDUFB8: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Belongs to the complex I NDUFB8 subunit family. Protein type: Endoplasmic reticulum; Energy Metabolism - oxidative phosphorylation; Oxidoreductase; EC 1.6.99.3; Membrane protein, integral; Mitochondrial; EC 1.6.5.3 Chromosomal Location of Human Ortholog: 10q24.31 Cellular Component: endoplasmic reticulum; mitochondrial inner membrane; integral to membrane; mitochondrial respiratory chain complex I Molecular Function: NADH dehydrogenase (ubiquinone) activity Biological Process: cellular metabolic process; mitochondrial electron transport, NADH to ubiquinone |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | O95169 |
| NCBI GenInfo Identifier: | 6093490 |
| NCBI Gene ID: | 4714 |
| NCBI Accession: | O95169.1 |
| UniProt Secondary Accession: | O95169 |
| UniProt Related Accession: | O95169 |
| Molecular Weight: | |
| NCBI Full Name: | NADH dehydrogenase |
| NCBI Synonym Full Names: | NADH:ubiquinone oxidoreductase subunit B8 |
| NCBI Official Symbol: | NDUFB8 |
| NCBI Official Synonym Symbols: | ASHI; CI-ASHI; MC1DN32 |
| NCBI Protein Information: | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial |
| UniProt Protein Name: | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial |
| UniProt Synonym Protein Names: | Complex I-ASHI; CI-ASHI; NADH-ubiquinone oxidoreductase ASHI subunit |
| Protein Family: | |
| UniProt Gene Name: | NDUFB8 |
| UniProt Entry Name: | NDUB8_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NDUFB8 Rabbit mAb (CAB19732) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s. |
 | Western blot analysis of extracts of various cell lines, using NDUFB8 Rabbit mAb (CAB19732) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s. |
 | Immunohistochemistry of paraffin-embedded human esophageal cancer using NDUFB8 Rabbit mAb (CAB19732) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse kidney using NDUFB8 Rabbit mAb (CAB19732) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat heart using NDUFB8 Rabbit mAb (CAB19732) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U-2 OS cells using NDUFB8 Rabbit mAb (CAB19732) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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