Cell Biology Antibodies 6
Anti-NDUFA4 Antibody (CAB15693)
(No reviews yet)
Write a Review
- SKU:
- CAB15693
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-NDUFA4 Antibody |
| Antibody SKU: | CAB15693 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-81 of human NDUFA4 (NP_002480.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-251MG, HepG2, HeLa, Mouse intestine, Mouse brain, Mouse heart, Mouse kidney, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-81 of human NDUFA4 (NP_002480.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MLRQ IIGQ AKKH PSLI PLFV FIGT GATG ATLY LLRL ALFN PDVC WDRN NPEP WNKL GPND QYKF YSVN VDYS KLKK ERPD F |
| Gene ID: | 4697 |
| Uniprot: | O00483 |
| Cellular Location: | Matrix side, Mitochondrion inner membrane, Peripheral membrane protein |
| Calculated MW: | 9kDa |
| Observed MW: | 12kDa |
| Synonyms: | NDUFA4, CI-9k, CI-MLRQ, MLRQ |
| Background: | The protein encoded by this gene belongs to the complex I 9kDa subunit family. Mammalian complex I of mitochondrial respiratory chain is composed of 45 different subunits. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. |
| UniProt Protein Function: | NDUFA4: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Belongs to the complex I NDUFA4 subunit family. |
| UniProt Protein Details: | Protein type:Oxidoreductase; Energy Metabolism - oxidative phosphorylation; EC 1.6.5.3; Mitochondrial; EC 1.6.99.3 Chromosomal Location of Human Ortholog: 7p21.3 Cellular Component: mitochondrial inner membrane; mitochondrial respiratory chain complex I; mitochondrial respiratory chain complex IV; mitochondrion Molecular Function:cytochrome-c oxidase activity; protein binding; protein complex binding Biological Process: mitochondrial electron transport, cytochrome c to oxygen |
| NCBI Summary: | The protein encoded by this gene belongs to the complex I 9kDa subunit family. Mammalian complex I of mitochondrial respiratory chain is composed of 45 different subunits. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O00483 |
| NCBI GenInfo Identifier: | 2499333 |
| NCBI Gene ID: | 4697 |
| NCBI Accession: | O00483.1 |
| UniProt Secondary Accession: | O00483,Q6FHN5, A4D109, |
| UniProt Related Accession: | O00483 |
| Molecular Weight: | 9,370 Da |
| NCBI Full Name: | Cytochrome c oxidase subunit NDUFA4 |
| NCBI Synonym Full Names: | NDUFA4, mitochondrial complex associated |
| NCBI Official Symbol: | NDUFA4 |
| NCBI Official Synonym Symbols: | MLRQ; CI-9k; CI-MLRQ |
| NCBI Protein Information: | cytochrome c oxidase subunit NDUFA4 |
| UniProt Protein Name: | Cytochrome c oxidase subunit NDUFA4 |
| UniProt Synonym Protein Names: | Complex I-MLRQ; CI-MLRQ; NADH-ubiquinone oxidoreductase MLRQ subunit |
| Protein Family: | Cytochrome c oxidase |
| UniProt Gene Name: | NDUFA4 |
| UniProt Entry Name: | NDUA4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NDUFA4 antibody (CAB15693) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of L929 cells using NDUFA4 Polyclonal Antibody (CAB15693) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using NDUFA4 Polyclonal Antibody (CAB15693) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
View AllClose
Related Products
![Anti-NDUFA4 Antibody (CAB19893)[KO Validated]](https://cdn11.bigcommerce.com/s-rd6ounxcu2/images/stencil/590x590/products/56064/61246/anti-ndufa4-antibody-cab19893ko-validated__91933__05201.1706533749.jpg?c=1 "Anti-NDUFA4 Antibody (CAB19893)[KO Validated]")
NDUFA4 Polyclonal Antibody
OverviewProduct Name:NDUFA4 Polyclonal AntibodyProduct Code:CAB21502Reactivity:Human, Mouse, RatApplications:Western blotting, Im
Human NDUFA4 Recombinant Protein (RPPB2004)
Enzymes Recombinant Proteins
system_update_altDatasheetsystem_update_altMSDS
