Name: Anti-NDUFA12 Antibody (CAB8237)
Brand: Cell Biology Antibodies 12
SKU: CAB8237
Availability: OutOfStock

Antibody Name:	NDUFA12 Rabbit Polyclonal Antibody
Antibody SKU:	CAB8237
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-145 of human NDUFA12 (NP_061326.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, PC-12, HepG2, Mouse kidney, Mouse heart, Mouse testis, Rat heart, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-145 of human NDUFA12 (NP_061326.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MELV QVLK RGLQ QITG HGGL RGYL RVFF RTND AKVG TLVG EDKY GNKY YEDN KQFF GRHR WVVY TTEM NGKN TFWD VDGS MVPP EWHR WLHS MTDD PPTT KPLT ARKF IWTN HKFN VTGT PEQY VPYS TTRK KIQE WIPP STPY K
Gene ID:	55967
Uniprot:	Q9UI09
Cellular Location:	Matrix side, Mitochondrion inner membrane, Peripheral membrane protein
Calculated MW:	7kDa/17kDa
Observed MW:	17kDa

Synonyms:

NDUFA12, B17.2, DAP13

Background:

This gene encodes a protein which is part of mitochondrial complex 1, part of the oxidative phosphorylation system in mitochondria. Complex 1 transfers electrons to ubiquinone from NADH which establishes a proton gradient for the generation of ATP. Mutations in this gene are associated with Leigh syndrome due to mitochondrial complex 1 deficiency. Pseudogenes of this gene are located on chromosomes 5 and 13. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	NDUFA12: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Defects in NDUFA12 are the cause of Leigh syndrome (LS). An early-onset progressive neurodegenerative disorder characterized by the presence of focal, bilateral lesions in one or more areas of the central nervous system including the brainstem, thalamus, basal ganglia, cerebellum and spinal cord. Clinical features depend on which areas of the central nervous system are involved and include subacute onset of psychomotor retardation, hypotonia, ataxia, weakness, vision loss, eye movement abnormalities, seizures, and dysphagia. Belongs to the complex I NDUFA12 subunit family.
UniProt Protein Details:	Protein type:Oxidoreductase; Mitochondrial; EC 1.6.99.3; EC 1.6.5.3 Chromosomal Location of Human Ortholog: 12q22 Cellular Component: mitochondrial inner membrane; mitochondrial respiratory chain complex I Biological Process: mitochondrial electron transport, NADH to ubiquinone; mitochondrial respiratory chain complex I assembly; response to oxidative stress Disease: Leigh Syndrome
NCBI Summary:	This gene encodes a protein which is part of mitochondrial complex 1, part of the oxidative phosphorylation system in mitochondria. Complex 1 transfers electrons to ubiquinone from NADH which establishes a proton gradient for the generation of ATP. Mutations in this gene are associated with Leigh syndrome due to mitochondrial complex 1 deficiency. Pseudogenes of this gene are located on chromosomes 5 and 13. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2012]
UniProt Code:	Q9UI09
NCBI GenInfo Identifier:	12229870
NCBI Gene ID:	55967
NCBI Accession:	Q9UI09.1
UniProt Secondary Accession:	Q9UI09,Q53XX0, Q9BRV6, F8VQS7,
UniProt Related Accession:	Q9UI09
Molecular Weight:	7,173 Da
NCBI Full Name:	NADH dehydrogenase
NCBI Synonym Full Names:	NADH:ubiquinone oxidoreductase subunit A12
NCBI Official Symbol:	NDUFA12
NCBI Official Synonym Symbols:	B17.2; DAP13
NCBI Protein Information:	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12
UniProt Protein Name:	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12
UniProt Synonym Protein Names:	13 kDa differentiation-associated protein; Complex I-B17.2; CI-B17.2; CIB17.2; NADH-ubiquinone oxidoreductase subunit B17.2
Protein Family:	NADH dehydrogenase
UniProt Gene Name:	NDUFA12
UniProt Entry Name:	NDUAC_HUMAN

Anti-NDUFA12 Polyclonal Antibody (CAB8237)

Western blot analysis of extracts of various cell lines, using NDUFA12 antibody (CAB8237) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Antibodies

NDUFA12 Antibody (PACO10774)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)