Name: Anti-NDRG1 Antibody (CAB18057)[KO Validated]
Brand: KO Validated Antibodies 1
SKU: CAB18057
Availability: OutOfStock

Antibody Name:	Anti-NDRG1 Antibody [KO Validated]
Antibody SKU:	CAB18057
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 175-394 of human NDRG1 (NP_001128714.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 175-394 of human NDRG1 (NP_001128714.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	WAAS KISG WTQA LPDM VVSH LFGK EEMQ SNVE VVHT YRQH IVND MNPG NLHL FINA YNSR RDLE IERP MPGT HTVT LQCP ALLV VGDS SPAV DAVV ECNS KLDP TKTT LLKM ADCG GLPQ ISQP AKLA EAFK YFVQ GMGY MPSA SMTR LMRS RTAS GSSV TSLD GTRS RSHT SEGT RSRS HTSE GTRS RSHT SEGA HLDI TPNS GAAG NSAG PKSM EVSC
Gene ID:	10397
Uniprot:	Q92597
Cellular Location:	Cell membrane, Cytoplasm, Nucleus, centrosome, cytoskeleton, cytosol, microtubule organizing center
Calculated MW:	33kDa/35kDa/42kDa
Observed MW:	48kDa

Anti-NDRG1 Antibody [KO Validated] (CAB18057)

Western blot analysis of extracts from normal (control) and NDRG1 knockout (KO) 293T cells, using NDRG1 antibody (CAB18057) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Synonyms:

NDRG1, CAP43, CMT4D, DRG-1, DRG1, GC4, HMSNL, NDR1, NMSL, PROXY1, RIT42, RTP, TARG1, TDD5

Background:

This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. The encoded protein is necessary for p53-mediated caspase activation and apoptosis. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

UniProt Protein Function:	NDRG1: a hypoxia-inducible anti-apoptotic protein. Promotes proliferation and correlates with invasion, metastasis and poor prognosis in many cancers. Is involved in growth arrest and cell differentiation during development. A ubiquitous Rab4a effector protein that modulates angiogenesis and is involved in vesicular recycling of E-cadherin. Colocalizes with transferrin during the recycling in cells. NDRG1 knockdown delays the recycling rate of transferrin, while its overexpression increases the rate of transferrin recycling. Interacts with SIRT1/p53 signaling to attenuate hypoxic injury in human trophoblasts. Its expression is induced by an elevation of free intracellular calcium ion caused by nickel ion exposure. Highly expressed in placental membranes, prostate, kidney, small intestine, ovary, peripheral nerves and Schwann cells. Defects in NDRG1 are the cause of Charcot-Marie-Tooth disease type 4D (CMT4D).
UniProt Protein Details:	Protein type:Cell development/differentiation; Vesicle Chromosomal Location of Human Ortholog: 8q24.3 Cellular Component: microtubule cytoskeleton; microtubule; centrosome; cell-cell adherens junction; recycling endosome membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; cytosol; nucleus Molecular Function:protein binding; gamma-tubulin binding; cadherin binding; microtubule binding; Rab GTPase binding Biological Process: response to metal ion; DNA damage response, signal transduction by p53 class mediator; myelin maintenance in the peripheral nervous system; mast cell activation Disease: Charcot-marie-tooth Disease, Type 4d
NCBI Summary:	This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. The encoded protein is necessary for p53-mediated caspase activation and apoptosis. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]
UniProt Code:	Q92597
NCBI GenInfo Identifier:	6166568
NCBI Gene ID:	10397
NCBI Accession:	Q92597.1
UniProt Secondary Accession:	Q92597,O15207, Q6IBG2, Q9NYR6, Q9UK29, B3KR80, B7Z446
UniProt Related Accession:	Q92597
Molecular Weight:	394
NCBI Full Name:	Protein NDRG1
NCBI Synonym Full Names:	N-myc downstream regulated 1
NCBI Official Symbol:	NDRG1
NCBI Official Synonym Symbols:	GC4; RTP; DRG1; NDR1; NMSL; TDD5; CAP43; CMT4D; DRG-1; HMSNL; RIT42; TARG1; PROXY1
NCBI Protein Information:	protein NDRG1; protein regulated by oxygen-1; differentiation-related gene 1 protein; nickel-specific induction protein Cap43; N-myc downstream-regulated gene 1 protein; reducing agents and tunicamycin-responsive protein
UniProt Protein Name:	Protein NDRG1
UniProt Synonym Protein Names:	Differentiation-related gene 1 protein; DRG-1; N-myc downstream-regulated gene 1 protein; Nickel-specific induction protein Cap43; Reducing agents and tunicamycin-responsive protein; RTP; Rit42
Protein Family:	Protein
UniProt Gene Name:	NDRG1
UniProt Entry Name:	NDRG1_HUMAN

Antibodies	ELISA Kits
Anti-NDRG1 Antibody (CAB2142)	Human NDRG1 ELISA Kit
Anti-Phospho-NDRG1-T346 pAb Antibody (CABP0792)	Human Protein NDRG1 (NDRG1) ELISA Kit
Anti-Phospho-NDRG1-S330 pAb Antibody (CABP0807)
Anti-NDRG1 Antibody (CAB4050)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)