Cell Cycle Antibodies 1

Anti-NDC80 Antibody (CAB5411)

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SKU:
CAB5411
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-NDC80 Antibody
Antibody SKU:CAB5411
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 343-642 of human NDC80 (NP_006092.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:K-562, Lovo, MCF7, 22Rv1, THP-1, 293T, Mouse testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 343-642 of human NDC80 (NP_006092.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ETIK QENT RLQN IIDN QKYS VADI ERIN HERN ELQQ TINK LTKD LEAE QQKL WNEE LKYA RGKE AIET QLAE YHKL ARKL KLIP KGAE NSKG YDFE IKFN PEAG ANCL VKYR AQVY VPLK ELLN ETEE EINK ALNK KMGL EDTL EQLN AMIT ESKR SVRT LKEE VQKL DDLY QQKI KEAE EEDE KCAS ELES LEKH KHLL ESTV NQGL SEAM NELD AVQR EYQL VVQT TTEE RRKV GNNL QRLL EMVA THVG SVEK HLEE QIAK VDRE YEEC MSED LSEN IKEI RDKY EKKA TLIK SSEE
Gene ID:10403
Uniprot:O14777
Cellular Location:Chromosome, Nucleus, centromere, kinetochore
Calculated MW:73kDa
Observed MW:80kDa
Synonyms:NDC80, HEC, HEC1, HsHec1, KNTC2, TID3, hsNDC80
Background:This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation.
UniProt Protein Function:HEC1: plays an essential role in chromosome segregation by interacting through its coiled-coil domains with several proteins that modulate the G2/M phase. Phosphorylation by Nek2 is essential for faithful chromosome segregation. Required for the recruitment of Mps1 kinase and Mad1/Mad2 complexes to kinetochores.
UniProt Protein Details:

Protein type:Cell cycle regulation

Chromosomal Location of Human Ortholog: 18p11.32

Cellular Component: chromosome, pericentric region; cytosol; kinetochore; membrane; nucleus

Molecular Function:identical protein binding; protein binding; structural constituent of cytoskeleton

Biological Process: attachment of spindle microtubules to kinetochore; attachment of spindle microtubules to kinetochore during mitosis; chromosome segregation; establishment of mitotic spindle orientation; mitosis; mitotic sister chromatid segregation; mitotic spindle organization and biogenesis; sister chromatid cohesion

NCBI Summary:This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011]
UniProt Code:O14777
NCBI GenInfo Identifier:74735330
NCBI Gene ID:10403
NCBI Accession:O14777.1
UniProt Secondary Accession:O14777,Q6PJX2,
UniProt Related Accession:O14777
Molecular Weight:73,913 Da
NCBI Full Name:Kinetochore protein NDC80 homolog
NCBI Synonym Full Names:NDC80, kinetochore complex component
NCBI Official Symbol:NDC80  
NCBI Official Synonym Symbols:HEC; HEC1; TID3; KNTC2; HsHec1; hsNDC80  
NCBI Protein Information:kinetochore protein NDC80 homolog
UniProt Protein Name:Kinetochore protein NDC80 homolog
UniProt Synonym Protein Names:Highly expressed in cancer protein; Kinetochore protein Hec1; HsHec1; Kinetochore-associated protein 2; Retinoblastoma-associated protein HEC
Protein Family:Kinetochore protein
UniProt Gene Name:NDC80  
UniProt Entry Name:NDC80_HUMAN
Anti-NDC80 Antibody (CAB5411)Western blot analysis of extracts of various cell lines, using NDC80 antibody (CAB5411) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
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