Cell Cycle Antibodies 2
Anti-NBN Antibody (CAB7703)
- SKU:
- CAB7703
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-NBN Antibody |
| Antibody SKU: | CAB7703 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide of human NBN |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Jurkat, HeLa, MCF7, Mouse heart, Rat kidney |
| Immunogen: | A synthetic peptide of human NBN |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 4683 |
| Uniprot: | O60934 |
| Cellular Location: | Chromosome, Nucleus, PML body, telomere |
| Calculated MW: | 84kDa |
| Observed MW: | 100kDa |
| Synonyms: | NBN, AT-V1, AT-V2, ATV, NBS, NBS1, P95, nibrin |
| Background: | Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. |
| UniProt Protein Function: | NBS1: a member of the MRE11/RAD50 double-strand break repair complex. Involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. Mutation results in the Nijmegen breakage syndrome (NBS), an autosomal recessive chromosomal instability syndrome. |
| UniProt Protein Details: | Protein type:Cell cycle regulation; DNA repair, damage Chromosomal Location of Human Ortholog: 8q21 Cellular Component: cytosol; Mre11 complex; nuclear chromosome, telomeric region; nuclear inclusion body; nucleolus; nucleoplasm; nucleus; PML body; replication fork Molecular Function:ATP-dependent DNA helicase activity; damaged DNA binding; protein binding; protein N-terminus binding; transcription factor binding Biological Process: blastocyst growth; cell cycle arrest; cell proliferation; DNA damage checkpoint; DNA damage response, signal transduction by p53 class mediator; DNA duplex unwinding; DNA repair; double-strand break repair; double-strand break repair via homologous recombination; double-strand break repair via nonhomologous end joining; double-strand break repair via synthesis-dependent strand annealing; isotype switching; meiotic cell cycle; mitotic cell cycle checkpoint; mitotic cell cycle G2/M transition DNA damage checkpoint; neuromuscular process controlling balance; positive regulation of kinase activity; positive regulation of protein amino acid autophosphorylation; positive regulation of telomere maintenance; regulation of DNA replication initiation; telomere maintenance; telomeric 3' overhang formation Disease: Aplastic Anemia; Leukemia, Acute Lymphoblastic; Nijmegen Breakage Syndrome |
| NCBI Summary: | Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O60934 |
| NCBI GenInfo Identifier: | 74762960 |
| NCBI Gene ID: | 4683 |
| NCBI Accession: | O60934.1 |
| UniProt Secondary Accession: | O60934,O60672, Q32NF7, Q53FM6, Q63HR6, Q7LDM2, B2R626 B2RNC5, |
| UniProt Related Accession: | O60934 |
| Molecular Weight: | 84,959 Da |
| NCBI Full Name: | Nibrin |
| NCBI Synonym Full Names: | nibrin |
| NCBI Official Symbol: | NBN |
| NCBI Official Synonym Symbols: | ATV; NBS; P95; NBS1; AT-V1; AT-V2 |
| NCBI Protein Information: | nibrin |
| UniProt Protein Name: | Nibrin |
| UniProt Synonym Protein Names: | Cell cycle regulatory protein p95; Nijmegen breakage syndrome protein 1 |
| Protein Family: | Nibrin |
| UniProt Gene Name: | NBN |
| UniProt Entry Name: | NBN_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NBN antibody (CAB7703) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 10s. |
 | Immunofluorescence analysis of U2OS cells using NBN antibody (CAB7703) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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