KO Validated Antibodies 1

Anti-NBN Antibody (CAB0783)[KO Validated]

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SKU:
CAB0783
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-NBN Antibody [KO Validated]
Antibody SKU:CAB0783
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 435-754 of human NBN (NP_002476.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:1000 IHC 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:293T, Mouse testis, Rat testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 435-754 of human NBN (NP_002476.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:KLPS INKS KDRA SQQQ QTNS IRNY FQPS TKKR ERDE ENQE MSSC KSAR IETS CSLL EQTQ PATP SLWK NKEQ HLSE NEPV DTNS DNNL FTDT DLKS IVKN SASK SHAA EKLR SNKK REMD DVAI EDEV LEQL FKDT KPEL EIDV KVQK QEED VNVR KRPR MDIE TNDT FSDE AVPE SSKI SQEN EIGK KREL KEDS LWSA KEIS NNDK LQDD SEML PKKL LLTE FRSL VIKN STSR NPSG INDD YGQL KNFK KFKK VTYP GAGK LPHI IGGS DLIA HHAR KNTE LEEW LRQE MEVQ NQHA KEES LADD LFRY NPYL KRRR
Gene ID:4683
Uniprot:O60934
Cellular Location:Chromosome, Nucleus, PML body, telomere
Calculated MW:84kDa
Observed MW:95kDa/70kDa
Synonyms:NBN, AT-V1, AT-V2, ATV, NBS, NBS1, P95, nibrin
Background:Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation.
UniProt Protein Function:NBS1: a member of the MRE11/RAD50 double-strand break repair complex. Involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. Mutation results in the Nijmegen breakage syndrome (NBS), an autosomal recessive chromosomal instability syndrome.
UniProt Protein Details:

Protein type:Cell cycle regulation; DNA repair, damage

Chromosomal Location of Human Ortholog: 8q21

Cellular Component: cytosol; Mre11 complex; nuclear chromosome, telomeric region; nuclear inclusion body; nucleolus; nucleoplasm; nucleus; PML body; replication fork

Molecular Function:ATP-dependent DNA helicase activity; damaged DNA binding; protein binding; protein N-terminus binding; transcription factor binding

Biological Process: blastocyst growth; cell cycle arrest; cell proliferation; DNA damage checkpoint; DNA damage response, signal transduction by p53 class mediator; DNA duplex unwinding; DNA repair; double-strand break repair; double-strand break repair via homologous recombination; double-strand break repair via nonhomologous end joining; double-strand break repair via synthesis-dependent strand annealing; isotype switching; meiotic cell cycle; mitotic cell cycle checkpoint; mitotic cell cycle G2/M transition DNA damage checkpoint; neuromuscular process controlling balance; positive regulation of kinase activity; positive regulation of protein amino acid autophosphorylation; positive regulation of telomere maintenance; regulation of DNA replication initiation; telomere maintenance; telomeric 3' overhang formation

Disease: Aplastic Anemia; Leukemia, Acute Lymphoblastic; Nijmegen Breakage Syndrome

NCBI Summary:Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. [provided by RefSeq, Jul 2008]
UniProt Code:O60934
NCBI GenInfo Identifier:74762960
NCBI Gene ID:4683
NCBI Accession:O60934.1
UniProt Secondary Accession:O60934,O60672, Q32NF7, Q53FM6, Q63HR6, Q7LDM2, B2R626 B2RNC5,
UniProt Related Accession:O60934
Molecular Weight:84,959 Da
NCBI Full Name:Nibrin
NCBI Synonym Full Names:nibrin
NCBI Official Symbol:NBN  
NCBI Official Synonym Symbols:ATV; NBS; P95; NBS1; AT-V1; AT-V2  
NCBI Protein Information:nibrin
UniProt Protein Name:Nibrin
UniProt Synonym Protein Names:Cell cycle regulatory protein p95; Nijmegen breakage syndrome protein 1
Protein Family:Nibrin
UniProt Gene Name:NBN  
UniProt Entry Name:NBN_HUMAN
Anti-NBN Antibody [KO Validated] (CAB0783)Western blot analysis of extracts of various cell lines, using NBN antibody (CAB0783) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-NBN Antibody [KO Validated] (CAB0783)Western blot analysis of extracts from normal (control) and NBN knockout (KO) 293T cells, using NBN antibody (CAB0783) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-NBN Antibody [KO Validated] (CAB0783)Immunohistochemistry of paraffin-embedded human lung cancer using [KO Validated] NBN Rabbit pAb (CAB0783) at dilution of 1:50 (40x lens).
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