The N-Cadherin Antibody (CAB0433) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the CDH2 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it well-suited for investigations in cell biology, developmental biology, and cancer research.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
N-Cadherin Antibody
SKU:
CAB0433
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Cell Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
100kDa
Observed MW:
140kDa
This gene encodes a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone.
Purification Method
Affinity purification
Gene ID
1000
RRID
AB_2757189
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse heart, using N-Cadherin Rabbit pAb (CAB0433) at 1:700 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Mouse heart using N-Cadherin Rabbit pAb (CAB0433) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
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