The Myosin heavy chain Monoclonal Antibody (CAB4963) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed in rabbits, is highly specific and reactive towards myosin heavy chain present in human samples, making it an excellent tool for studying muscle physiology and function.Myosin heavy chain is a critical component of muscle fibers, contributing to their structure and contractile activity. Research on this protein is essential for understanding muscle development, function, and diseases such as muscular dystrophy and heart failure. The Myosin Heavy Chain Rabbit Monoclonal Antibody enables the detection and analysis of myosin heavy chain in various cell types, making it invaluable for studies in physiology, pharmacology, and muscle-related disorders.
This antibody is validated for use in WB, IHC-P, ELISA, IF-P applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
Myosin heavy chain Monoclonal Antibody
SKU:
CAB4963
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Clone Number:
ARC1170
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CMD1S, CMH1, MPD1, MYHCB, SPMD, SPMM, Myosin heavy chain
Positive Sample:
Mouse lung, Mouse skeletal muscle, Rat heart
Cellular Localization:
Cytoplasm, Myofibril.
Calculated MW:
224kDa
Observed MW:
224kDa
Muscle myosin is a hexameric protein containing 2 heavy chain subunits, 2 alkali light chain subunits, and 2 regulatory light chain subunits. This gene encodes the beta (or slow) heavy chain subunit of cardiac myosin. It is expressed predominantly in normal human ventricle. It is also expressed in skeletal muscle tissues rich in slow-twitch type I muscle fibers. Changes in the relative abundance of this protein and the alpha (or fast) heavy subunit of cardiac myosin correlate with the contractile velocity of cardiac muscle. Its expression is also altered during thyroid hormone depletion and hemodynamic overloading. Mutations in this gene are associated with familial hypertrophic cardiomyopathy, myosin storage myopathy, dilated cardiomyopathy, and Laing early-onset distal myopathy. [provided by RefSeq, Jul 2008]
Purification Method
Affinity purification
Gene ID
4625 4624
RRID
AB_2863399
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Myosin heavy chain Rabbit mAb (CAB4963) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Mouse heart using Myosin heavy chain Rabbit mAb (CAB4963) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of paraffin-embedded rat heart using Myosin heavy chain Rabbit mAb (CAB4963) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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