Cell Biology Antibodies 6
Anti-MYOF Antibody (CAB15427)
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- SKU:
- CAB15427
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-MYOF Antibody |
| Antibody SKU: | CAB15427 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human MYOF (NP_038479.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:200 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | U-251MG, A-549, HeLa, NIH/3T3 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human MYOF (NP_038479.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MLRV IVES ASNI PKTK FGKP DPIV SVIF KDEK KKTK KVDN ELNP VWNE ILEF DLRG IPLD FSSS LGII VKDF ETIG QNKL IGTA TVAL KDLT GDQS RSLP YKLI SLLN EKGQ DTGA TIDL VIGY DPPS APHP NDLS GPSV PGMG GDGE EDEG DEDR LDNA VRGP GPKG PVGT VSEA QLAR RLTK VKNS RR |
| Gene ID: | 26509 |
| Uniprot: | Q9NZM1 |
| Cellular Location: | Cell membrane, Cytoplasmic vesicle membrane, Nucleus membrane, Single-pass type II membrane protein |
| Calculated MW: | 17kDa/46kDa/49kDa/179kDa/229kDa/233kDa/234kDa |
| Observed MW: | 250kDa |
| Synonyms: | MYOF, FER1L3, myoferlin |
| Background: | Mutations in dysferlin, a protein associated with the plasma membrane, can cause muscle weakness that affects both proximal and distal muscles. The protein encoded by this gene is a type II membrane protein that is structurally similar to dysferlin. It is a member of the ferlin family and associates with both plasma and nuclear membranes. The protein contains C2 domains that play a role in calcium-mediated membrane fusion events, suggesting that it may be involved in membrane regeneration and repair. Two transcript variants encoding different isoforms have been found for this gene. Other possible variants have been detected, but their full-length nature has not been determined. |
| UniProt Protein Function: | myoferlin: Calcium/phospholipid-binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. Involved in endocytic recycling. Implicated in VEGF signal transduction by regulating the levels of the receptor KDR. Belongs to the ferlin family. 8 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Cell surface Chromosomal Location of Human Ortholog: 10q24 Cellular Component: nuclear membrane; cytoplasmic vesicle membrane; intracellular membrane-bound organelle; integral to membrane; plasma membrane; nuclear envelope; caveola; cytoplasmic vesicle Molecular Function:protein binding; phospholipid binding Biological Process: muscle contraction; plasma membrane repair; blood circulation; regulation of vascular endothelial growth factor receptor signaling pathway; myoblast fusion |
| NCBI Summary: | Mutations in dysferlin, a protein associated with the plasma membrane, can cause muscle weakness that affects both proximal and distal muscles. The protein encoded by this gene is a type II membrane protein that is structurally similar to dysferlin. It is a member of the ferlin family and associates with both plasma and nuclear membranes. The protein contains C2 domains that play a role in calcium-mediated membrane fusion events, suggesting that it may be involved in membrane regeneration and repair. Two transcript variants encoding different isoforms have been found for this gene. Other possible variants have been detected, but their full-length nature has not been determined. [provided by RefSeq, Dec 2008] |
| UniProt Code: | Q9NZM1 |
| NCBI GenInfo Identifier: | 20139241 |
| NCBI Gene ID: | 26509 |
| NCBI Accession: | Q9NZM1.1 |
| UniProt Related Accession: | Q9NZM1 |
| Molecular Weight: | Predicted MW: 62.4kDaAccurate MW: 62/44kDa |
| NCBI Full Name: | Myoferlin |
| NCBI Synonym Full Names: | myoferlin |
| NCBI Official Symbol: | MYOF |
| NCBI Official Synonym Symbols: | FER1L3 |
| NCBI Protein Information: | myoferlin |
| UniProt Protein Name: | Myoferlin |
| UniProt Synonym Protein Names: | Fer-1-like protein 3 |
| Protein Family: | Myosin |
| UniProt Gene Name: | MYOF |
| UniProt Entry Name: | MYOF_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MYOF antibody (CAB15427) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 2s. |
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