Developmental Biology

Anti-MYF6 Antibody (CAB15291)

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SKU:
CAB15291
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Developmental Biology

Description

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Antibody Name:Anti-MYF6 Antibody
Antibody SKU:CAB15291
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 80-180 of human MYF6 (NP_002460.1).
Application:WB IHC
Recommended Dilution:WB 1:200 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HepG2, Mouse skeletal muscle, Mouse spleen, Rat skeletal muscle
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 80-180 of human MYF6 (NP_002460.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:WACK TCKR KSAP TDRR KAAT LRER RRLK KINE AFEA LKRR TVAN PNQR LPKV EILR SAIS YIER LQDL LHRL DQQE KMQE LGVD PFSY RPKQ ENLE GADF L
Gene ID:4618
Uniprot:P23409
Cellular Location:Nucleus
Calculated MW:26kDa
Observed MW:27kDa
Synonyms:MYF6, CNM3, MRF4, bHLHc4, myf-6
Background:The protein encoded by this gene is a probable basic helix-loop-helix (bHLH) DNA binding protein involved in muscle differentiation. The encoded protein likely acts as a heterodimer with another bHLH protein. Defects in this gene are a cause of autosomal dominant centronuclear myopathy (ADCNM).
UniProt Protein Function:Myf-6: Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein. Defects in MYF6 may be a cause of centronuclear myopathy type 3 (CNM3). A congenital muscle disorder characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. It may also affect distal muscles. Weakness may be present during childhood or adolescence or may not become evident until the third decade of life. Ptosis is a frequent clinical feature. The most prominent histopathologic features include high frequency of centrally located nuclei in muscle fibers not secondary to regeneration, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers.
UniProt Protein Details:

Protein type:DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 12q21

Cellular Component: nucleoplasm; nucleus

Molecular Function:RNA polymerase II transcription factor activity, enhancer binding; protein heterodimerization activity; transcription factor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; muscle cell differentiation; skeletal muscle development; somitogenesis; positive regulation of skeletal muscle fiber development; muscle cell fate commitment; positive regulation of muscle cell differentiation; skeletal muscle regeneration; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of myoblast differentiation

Disease: Myopathy, Centronuclear, 3

NCBI Summary:The protein encoded by this gene is a probable basic helix-loop-helix (bHLH) DNA binding protein involved in muscle differentiation. The encoded protein likely acts as a heterodimer with another bHLH protein. Defects in this gene are a cause of autosomal dominant centronuclear myopathy (ADCNM). [provided by RefSeq, May 2010]
UniProt Code:P23409
NCBI GenInfo Identifier:4505299
NCBI Gene ID:4618
NCBI Accession:NP_002460.1
UniProt Secondary Accession:P23409,Q53X80, Q6FHI9, B2R898,
UniProt Related Accession:P23409
Molecular Weight:
NCBI Full Name:myogenic factor 6
NCBI Synonym Full Names:myogenic factor 6 (herculin)
NCBI Official Symbol:MYF6  
NCBI Official Synonym Symbols:CNM3; MRF4; myf-6; bHLHc4  
NCBI Protein Information:myogenic factor 6
UniProt Protein Name:Myogenic factor 6
UniProt Synonym Protein Names:Class C basic helix-loop-helix protein 4; bHLHc4; Muscle-specific regulatory factor 4
Protein Family:Myogenic factor
UniProt Gene Name:MYF6  
UniProt Entry Name:MYF6_HUMAN
Anti-MYF6 Antibody (CAB15291)Western blot analysis of extracts of various cell lines, using MYF6 antibody (CAB15291) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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