Epigenetics & Nuclear Signaling Antibodies 2

Anti-MXD4 Antibody (CAB16485)

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SKU:
CAB16485
Product Type:
Antibody
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-MXD4 Antibody
Antibody SKU:CAB16485
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 110-209 of human MXD4 (NP_006445.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse
Positive Samples:Mouse lung
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 110-209 of human MXD4 (NP_006445.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:RRAL SIKE QLQQ EHRF LKRR LEQL SVQS VERV RTDS TGSA VSTD DSEQ EVDI EGME FGPG ELDS VGSS SDAD DHYS LQSG TGGD SGFG PHCR RLGR PALS
Gene ID:10608
Uniprot:Q14582
Cellular Location:Nucleus
Calculated MW:23kDa
Observed MW:30kDa
Synonyms:MXD4, MAD4, MST149, MSTP149, bHLHc12
Background:This gene is a member of the MAD gene family . The MAD genes encode basic helix-loop-helix-leucine zipper proteins that heterodimerize with MAX protein, forming a transcriptional repression complex. The MAD proteins compete for MAX binding with MYC, which heterodimerizes with MAX forming a transcriptional activation complex. Studies in rodents suggest that the MAD genes are tumor suppressors and contribute to the regulation of cell growth in differentiating tissues.
UniProt Protein Function:Mad4: Transcriptional repressor. Binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. Antagonizes MYC transcriptional activity by competing for MAX and suppresses MYC dependent cell transformation.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 4p16.3

Cellular Component: nucleus

Molecular Function:DNA binding; protein binding; protein dimerization activity; transcription corepressor activity

Biological Process: negative regulation of cell proliferation; negative regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent

NCBI Summary:This gene is a member of the MAD gene family . The MAD genes encode basic helix-loop-helix-leucine zipper proteins that heterodimerize with MAX protein, forming a transcriptional repression complex. The MAD proteins compete for MAX binding with MYC, which heterodimerizes with MAX forming a transcriptional activation complex. Studies in rodents suggest that the MAD genes are tumor suppressors and contribute to the regulation of cell growth in differentiating tissues. [provided by RefSeq, Jul 2008]
UniProt Code:Q14582
NCBI GenInfo Identifier:5453734
NCBI Gene ID:10608
NCBI Accession:NP_006445.1
UniProt Secondary Accession:Q14582,Q5TZX4, A2A335,
UniProt Related Accession:Q14582
Molecular Weight:23,528 Da
NCBI Full Name:max dimerization protein 4
NCBI Synonym Full Names:MAX dimerization protein 4
NCBI Official Symbol:MXD4  
NCBI Official Synonym Symbols:MAD4; MST149; MSTP149; bHLHc12  
NCBI Protein Information:max dimerization protein 4
UniProt Protein Name:Max dimerization protein 4
UniProt Synonym Protein Names:Class C basic helix-loop-helix protein 12; bHLHc12; Max-associated protein 4; Max-interacting transcriptional repressor MAD4
Protein Family:Max dimerization protein
UniProt Gene Name:MXD4  
UniProt Entry Name:MAD4_HUMAN
Anti-MXD4 Antibody (CAB16485)Western blot analysis of extracts of mouse lung, using MXD4 antibody (CAB16485) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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