Anti-MUTA Antibody (CAB9339)
- SKU:
- CAB9339
- Product Type:
- Antibody
- Antibody Type:
- Monoclonal Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
Description
| Antibody Name: | Anti-MUTA Antibody (CAB9339) |
| Antibody SKU: | CAB9339 |
| Antibody Size: | 50µL, 100µL |
| Application: | Western blotting, Immunohistochemistry, Immunofluorescence |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthesized peptide derived from human MUTA. |
| Application: | Western blotting, Immunohistochemistry, Immunofluorescence |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, 293T, HepG2, Mouse liver, Mouse kidney, Mouse heart, Rat liver, Rat kidney, Rat heart |
| Immunogen: | A synthesized peptide derived from human MUTA. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Cellular Location: | Mitochondrion matrix |
| Calculated MW: | 78kDa |
| Observed MW: | 83KDa |
| Synonyms: | MUT, MCM, methylmalonyl-CoA mutase |
| Background: | This gene encodes the mitochondrial enzyme methylmalonyl Coenzyme A mutase. In humans, the product of this gene is a vitamin B12-dependent enzyme which catalyzes the isomerization of methylmalonyl-CoA to succinyl-CoA, while in other species this enzyme may have different functions. Mutations in this gene may lead to various types of methylmalonic aciduria. |
 | Immunofluorescence analysis of HepG2 cells using MUTA antibody at dilution of 1:150. Blue: DAPI for nuclear staining. |
 | Immunohistochemistry of paraffin-embedded human liver using MUTA Rabbit mAb at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. |
 | Western blot analysis of extracts of various cell lines, using at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human colon carcinoma using MUTA Rabbit mAb at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. |
 | Western blot analysis of extracts of various cell lines, using at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. |
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