Antibody Name:	Anti-MTIF2 Antibody
Antibody SKU:	CAB16402
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 428-727 of human MTIF2 (NP_002444.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	LO2, A-431, U-251MG, Mouse heart, Mouse kidney, Mouse liver, Mouse spleen, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 428-727 of human MTIF2 (NP_002444.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GEEI LEVE SEPR AREV VDWR KYEQ EQEK GQED LKII EEKR KEHK EAHQ KARE KYGH LLWK KRSI LRFL ERKE QIPL KPKE KRER DSNV LSVI IKGD VDGS VEAI LNII DTYD ASHE CELE LVHF GVGD VSAN DVNL AETF DGVI YGFN VNAG NVIQ QSAA KKGV KIKL HKII YRLV EDLQ EELS SRLP CAVE EHPV GEAS ILAT FSVT EGKK KVPV AGCR VQKG QLEK QKKF KLTR NGHV IWKG SLTS LKHH KDDI SIVK TGMD CGLS LDED NMEF QVGD RIVC YEEK QIQA KTSW DPGF
Gene ID:	4528
Uniprot:	P46199
Cellular Location:	Mitochondrion
Calculated MW:	81kDa
Observed MW:	81kDa

Synonyms:

MTIF2

Background:

During the initiation of protein biosynthesis, initiation factor-2 (IF-2) promotes the binding of the initiator tRNA to the small subunit of the ribosome in a GTP-dependent manner. Prokaryotic IF-2 is a single polypeptide, while eukaryotic cytoplasmic IF-2 (eIF-2) is a trimeric protein. Bovine liver mitochondria contain IF-2(mt), an 85-kD monomeric protein that is equivalent to prokaryotic IF-2. The predicted 727-amino acid human protein contains a 29-amino acid presequence. Human IF-2(mt) shares 32 to 38% amino acid sequence identity with yeast IF-2(mt) and several prokaryotic IF-2s, with the greatest degree of conservation in the G domains of the proteins.

UniProt Protein Function:	MTIF2: One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex. Belongs to the IF-2 family.
UniProt Protein Details:	Protein type:Translation; Translation initiation; Mitochondrial Chromosomal Location of Human Ortholog: 2p16.1 Cellular Component: mitochondrion; nucleoplasm Molecular Function:GTPase activity; ribosomal small subunit binding; translation factor activity, nucleic acid binding Biological Process: regulation of translational initiation; ribosome disassembly
NCBI Summary:	During the initiation of protein biosynthesis, initiation factor-2 (IF-2) promotes the binding of the initiator tRNA to the small subunit of the ribosome in a GTP-dependent manner. Prokaryotic IF-2 is a single polypeptide, while eukaryotic cytoplasmic IF-2 (eIF-2) is a trimeric protein. Bovine liver mitochondria contain IF-2(mt), an 85-kD monomeric protein that is equivalent to prokaryotic IF-2. The predicted 727-amino acid human protein contains a 29-amino acid presequence. Human IF-2(mt) shares 32 to 38% amino acid sequence identity with yeast IF-2(mt) and several prokaryotic IF-2s, with the greatest degree of conservation in the G domains of the proteins. [provided by RefSeq, Mar 2016]
UniProt Code:	P46199
NCBI GenInfo Identifier:	223590069
NCBI Gene ID:	4528
NCBI Accession:	P46199.2
UniProt Secondary Accession:	P46199,D6W5D0,
UniProt Related Accession:	P46199
Molecular Weight:	81,317 Da
NCBI Full Name:	Translation initiation factor IF-2, mitochondrial
NCBI Synonym Full Names:	mitochondrial translational initiation factor 2
NCBI Official Symbol:	MTIF2
NCBI Protein Information:	translation initiation factor IF-2, mitochondrial
UniProt Protein Name:	Translation initiation factor IF-2, mitochondrial
UniProt Gene Name:	MTIF2
UniProt Entry Name:	IF2M_HUMAN

	Western blot analysis of extracts of various cell lines, using MTIF2 antibody (CAB16402) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded human lung cancer using MTIF2 antibody (CAB16402) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon carcinoma using MTIF2 antibody (CAB16402) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using MTIF2 Polyclonal Antibody (CAB16402) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using MTIF2 Polyclonal Antibody (CAB16402) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.