KO Validated Antibodies 2

Anti-MTA2 [KO Validated] Antibody (CAB4624)

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SKU:
CAB4624
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Applications:
WB
Applications:
IHC
Applications:
IF
Applications:
IP
Applications:
ChIP
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
MTA1L1
Synonyms:
PID
Research Area:
Cell Biology

Description

Product Name:[KO Validated] MTA2 Rabbit mAb
Product Code:CAB4624
Size:20uL, 50uL, 100uL
Synonyms:MTA1L1, PID
Applications:WB, IHC, IF, IP, ChIP
Reactivity:Human
Host Species:Rabbit
Immunogen:A synthesized peptide derived from human MTA2
Applications:WB, IHC, IF, IP, ChIP
Recommended Dilutions:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 ChIP 1:20 - 1:200
Reactivity:Human
Positive Samples:293T
Immunogen:A synthesized peptide derived from human MTA2
Purification Method:Affinity purification
Storage:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:9219
Uniprot:O94776
Cellular Location:Nucleus
Calculated MW:75kDa
Observed MW:75KDa
UniProt Protein Function:MTA2: May be involved in the regulation of gene expression as repressor and activator. The repression might be related to covalent modification of histone proteins.
UniProt Protein Details:

Protein type:Transcription factor; DNA-binding; Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Deacetylase

Chromosomal Location of Human Ortholog: 11q12-q13.1

Cellular Component: nucleoplasm; transcription factor complex; protein complex; membrane; histone deacetylase complex; nuclear chromatin; NuRD complex

Molecular Function:protein binding; nucleosomal DNA binding; zinc ion binding; histone deacetylase activity; transcription factor activity

Biological Process: chromatin assembly or disassembly; establishment and/or maintenance of chromatin architecture; ATP-dependent chromatin remodeling; DNA methylation; positive regulation of transcription from RNA polymerase II promoter; histone deacetylation; negative regulation of transcription from RNA polymerase II promoter

NCBI Summary:This gene encodes a protein that has been identified as a component of NuRD, a nucleosome remodeling deacetylase complex identified in the nucleus of human cells. It shows a very broad expression pattern and is strongly expressed in many tissues. It may represent one member of a small gene family that encode different but related proteins involved either directly or indirectly in transcriptional regulation. Their indirect effects on transcriptional regulation may include chromatin remodeling. It is closely related to another member of this family, a protein that has been correlated with the metastatic potential of certain carcinomas. These two proteins are so closely related that they share the same types of domains. These domains include two DNA binding domains, a dimerization domain, and a domain commonly found in proteins that methylate DNA. One of the proteins known to be a target protein for this gene product is p53. Deacetylation of p53 is correlated with a loss of growth inhibition in transformed cells supporting a connection between these gene family members and metastasis. [provided by RefSeq, May 2011]
UniProt Code:O94776
NCBI GenInfo Identifier:29840793
NCBI Gene ID:9219
NCBI Accession:O94776.1
UniProt Related Accession:O94776
Molecular Weight:Observed Molecular Weight: (Observed)75kD.Predicted Molecular Weight: (Calculated)75kDa.
NCBI Full Name:Metastasis-associated protein MTA2
NCBI Synonym Full Names:metastasis associated 1 family member 2
NCBI Official Symbol:MTA2  
NCBI Official Synonym Symbols:PID; MTA1L1  
NCBI Protein Information:metastasis-associated protein MTA2
UniProt Protein Name:Metastasis-associated protein MTA2
UniProt Synonym Protein Names:Metastasis-associated 1-like 1; MTA1-L1 protein; p53 target protein in deacetylase complex
Protein Family:Metastasis-associated protein
UniProt Gene Name:MTA2  
UniProt Entry Name:MTA2_HUMAN
Western blot - [KO Validated] MTA2 Rabbit mAb Western blot analysis of extracts of various cell lines, using MTA2 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Western blot - [KO Validated] MTA2 Rabbit mAb Western blot analysis of extracts of 293T cells, using MTA2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry - [KO Validated] MTA2 Rabbit mAb Immunohistochemistry of paraffin-embedded human colon using MTA2 Rabbit mAb at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with IHC staining protocol.
Immunoprecipitation - [KO Validated] MTA2 Rabbit mAb Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug [KO Validated] MTA2 antibody at a dilition of 1:500.
Chromatin Immunoprecipitation - [KO Validated] MTA2 Rabbit mAb Chromatin immunoprecipitation analysis of extracts of HeLa cells, using MTA2 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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