Metabolism Antibodies 2
Anti-MT-ATP8 Antibody (CAB17890)
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- SKU:
- CAB17890
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-MT-ATP8 Antibody |
| Antibody SKU: | CAB17890 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant protein of human MT-ATP8. |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, Jurkat, HepG2, A-549 |
| Immunogen: | Recombinant protein of human MT-ATP8. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 4509 |
| Uniprot: | P03928 |
| Cellular Location: | |
| Calculated MW: | 8kDa |
| Observed MW: | 8kDa |
| Synonyms: | ATPase8, MTATP8, ATP8, MT-ATP8 |
| Background: |
| UniProt Protein Function: | ATP8: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane. Belongs to the ATPase protein 8 family. |
| UniProt Protein Details: | Protein type:Hydrolase; EC 3.6.3.14; Mitochondrial; Transporter; Membrane protein, integral Chromosomal Location of Human Ortholog: - |
| UniProt Code: | P03928 |
| NCBI GenInfo Identifier: | 251831111 |
| NCBI Gene ID: | 4509 |
| NCBI Accession: | YP_003024030.1 |
| UniProt Secondary Accession: | P03928,Q34771, |
| Molecular Weight: | 7,992 Da |
| NCBI Full Name: | ATP synthase F0 subunit 8 (mitochondrion) |
| NCBI Synonym Full Names: | mitochondrially encoded ATP synthase 8 |
| NCBI Official Symbol: | MT-ATP8 |
| NCBI Official Synonym Symbols: | ATPase8; MTATP8; ATP8 |
| NCBI Protein Information: | ATP synthase F0 subunit 8 |
| UniProt Protein Name: | ATP synthase protein 8 |
| UniProt Synonym Protein Names: | A6L; F-ATPase subunit 8 |
| UniProt Gene Name: | MT-ATP8 |
| UniProt Entry Name: | ATP8_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MT-ATP8 antibody (CAB17890) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 2min. |
 | Immunofluorescence analysis of HeLa cells using MT-ATP8 antibody (CAB17890) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of HeLa cells using MT-ATP8 antibody (CAB17890) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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