Product Name:	Anti-Mouse IFNβ In Vivo Antibody - Low Endotoxin
Product Code:	IVMB0175
Size:	1mg, 5mg, 25mg, 50mg, 100mg
Clone:	MIB-5E9.1
Protein:	IFNβ
Product Type:	Monoclonal Antibody
Synonyms:	Interferon-β, Fibroblast interferon, Type-1 interferon, acid-stable interferon, interferon beta
Isotype:	Armenian Hamster IgG
Reactivity:	Mouse
Applications:	N, WB
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC >95% by SDS Page
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Applications:	N, WB
Recommended Usage:	WB The purified antibody is recommended for Western blotting. The suggested concentration for this MIB-5E9.1 antibody for use in western blotting is 1-10 µg/ml.
Reactivity:	Mouse

Host Species:	Armenian Hamster
Specificity:	Armenian Hamster Anti-Mouse IFNβ (Clone MIB-5E9.1) recognizes Mouse IFNβ. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution:	Fibroblasts & epithelial cells
Concentration:	≥ 5.0 mg/ml
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC >95% by SDS Page
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Interferons (IFN) consist of two classes. The type I IFN class consists of 20 highly similar IFN-α proteins and a single IFN-β protein. The second class, type II IFN, consists of a single protein, IFN-γ. IFN-α and IFN-β signal through the same cell surface receptor IFNAR1 and have a similar range of biological activities including antiviral and antiproliferative activity. The type I IFNs also influence activation, growth and differentiation of T cells, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. IFN-β has recently proven to be beneficial in patients with multiple sclerosis and could also be a potential therapy for rheumatoid arthritis. It is unclear what cellular mechanisms are involved in IFN-β’s therapeutic effects, but they may be caused by a restoration of balance between pro- and anti-inflammatory cytokines.

Technical Datasheet:	View
Protein:	IFNβ
Ligand/Receptor:	IFNα/βR (IFNAR-1, IFNAR-2)
Research Area:	Apoptosis, Cell Biology, Cell Death, Signal Transduction, Tumor Suppressors