Anti-Mouse CD4 [GK1.5] In Vivo Antibody - Ultra Low Endotoxin
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![Anti-Mouse CD4 [GK1.5] In Vivo Antibody - Ultra Low Endotoxin](https://cdn11.bigcommerce.com/s-rd6ounxcu2/images/stencil/608x608/products/78707/83889/anti-mouse-cd4-gk1.5-in-vivo-antibody-ultra-low-endotoxin__92374__56389.1706545321.jpg?c=1 "Anti-Mouse CD4 [GK1.5] In Vivo Antibody - Ultra Low Endotoxin")
- SKU:
- IVMB0061
- Product Type:
- In Vivo Monoclonal Antibody
- Clone:
- GK1.5
- Protein:
- CD4
- Isotype:
- Rat IgG2b kappa
- Reactivity:
- Mouse
- Synonyms:
- L3T4
- Synonyms:
- T4
- Research Area:
- CD4+ T Cell Depletion
- Endotoxin Level:
- Ultra Low Endotoxin
- Host Species:
- Rat
- Applications:
- Blocking
- Applications:
- Costim
- Applications:
- CyTOF
- Applications:
- Depletion
- Applications:
- FA
- Applications:
- FC
- Applications:
- IHC
- Applications:
- IP
![Anti-Mouse CD4 [GK1.5] In Vivo Antibody - Ultra Low Endotoxin](https://cdn11.bigcommerce.com/s-rd6ounxcu2/images/stencil/1280x1280/products/78707/83889/anti-mouse-cd4-gk1.5-in-vivo-antibody-ultra-low-endotoxin__92374__56389.1706545321.jpg?c=1&imbypass=on "Anti-Mouse CD4 [GK1.5] In Vivo Antibody - Ultra Low Endotoxin")
Description
system_update_altTechnical Datasheet
| Product Name: | Anti-Mouse CD4 [GK1.5] In Vivo Antibody - Ultra Low Endotoxin |
| Product Code: | IVMB0061 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | GK1.5 |
| Protein: | CD4 |
| Product Type: | Monoclonal Antibody |
| Synonyms: | L3T4, T4 |
| Isotype: | Rat IgG2b κ |
| Reactivity: | Mouse |
| Immunogen: | Mouse CTL clone V4 |
| Applications: | B, Costim, CyTOF®, Depletion, FA, FC, IHC, IP |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | <0.5 EU/mg as determined by the LAL method |
| Purity: | ≥98% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | B, Costim, CyTOF®, Depletion, FA, FC, IHC, IP |
| Recommended Usage: | CyTOF®FC The suggested concentration for this GK1.5 antibody for staining cells in flow cytometry is ≤ 1.0 µg per 106 in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application. |
| Reactivity: | Mouse |
| Host Species: | Rat |
| Specificity: | Rat Anti-Mouse CD4 (Clone GK1.5) recognizes an epitope on Mouse CD4. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody was also pathogen tested and third-party certified by IDEXX BioReseach to meet the lowest mycoplasma specification and free of any viral pathogens of concern. |
| Antigen Distribution: | The CD4/L3T4 antigen is expressed by the helper/inducer subset of mouse T-cells. The antigen is present on approximately 80% of thymocytes, 20% of spleen cells and 60% of lymph node cells. The expression of L3T4 correlates with class II MHC antigen reactivity on cloned T-cell lines. |
| Immunogen: | Mouse CTL clone V4 |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | <0.5 EU/mg as determined by the LAL method |
| Purity: | ≥98% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. CD4 interacts with class II molecules of the major histocompatibility complex (MHC) enhancing the signal for T-cell activation.6
| Technical Datasheet: | View |
| Protein: | CD4 |
| Ligand/Receptor: | MHC class II molecule |
| Research Area: | Immunology |
| Rat IgG2b Isotype Control | |
|---|---|
| Clone | 1-2 |
| Isotype | Rat IgG2b kappa |
| Endotoxin Level | Ultra Low Endotoxin |
Meet the team!
Shane Costigan
Territory Manager & Team Lead
Abdul Khadim
Sales Executive
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