Product Name:	Anti-Mouse CD366 (Tim-3) In Vivo Antibody - Low Endotoxin
Product Code:	IVMB0097
Size:	1mg, 5mg, 25mg, 50mg, 100mg
Clone:	RMT3-23
Protein:	Tim-3
Product Type:	Monoclonal Antibody
Synonyms:	Havcr2
Isotype:	Rat IgG2a κ
Reactivity:	Mouse
Immunogen:	This antibody was produced using recombinant mouse TIM-3
Applications:	B, FC, IF Staining, IHC, IHC FF, PhenoCycler®
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC >95% by SDS Page
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Applications:	B, CODEX®, FC, IF Staining, IHC, IHC FF
Recommended Usage:	FC The suggested concentration for CD366 antibody (clone RMT3-23) for staining cells in flow cytometry is ≤ 1.0 µg per 10⁶ in a volume of 100 µl or 100µl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Reactivity:	Mouse

Host Species:	Rat
Specificity:	Clone RMT2-23 recognizes an epitope on mouse Tim-3.
Antigen Distribution:	Tim-3 is expressed on activated Th1 and Tc1 lymphocytes, CD11b+ macrophages, Tregs, NK cells, and mast cells.
Immunogen:	This antibody was produced using recombinant mouse TIM-3
Concentration:	≥ 5.0 mg/ml
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC >95% by SDS Page
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Tim-3 antibody, clone RMT3-23, recognizes T cell immunoglobulin and mucin domain-containing protein 3 (TIM3, also known as CD366), a TIM family member of immunoregulatory proteins. Tim-3 is a 60kDa type I transmembrane protein with an extracellular immunoglobulin and mucin-like domain and a cytoplasmic tyrosine phosphorylation motif. Tim-3 is expressed by IFNg-producing Th1 CD4 and Tc1 (cytotoxic) CD8 T cells1, regulatory T cells (Tregs)2, myeloid cells3, NK cells4, and mast cells5. Tim-3 is an inhibitory molecule that limits Th1-mediated inflammatory diseases, including a model of central nervous system inflammation (experimental autoimmune encephalomyelitis, EAE)1, inflammatory bowel disease (IBD)6, and type I diabetes7. Tim-3 is also proposed to induce immunological tolerance7, promote Th1 apoptosis8, and regulate macrophage activation1. In addition, Tim-3 is upregulated on tumor-infiltrating lymphocytes, and coblockade of Tim-3 and immune checkpoint inhibitors, such as PD-1, is currently being investigated in clinical trials for the treatment of cancer9.

Technical Datasheet:	View
Protein:	Tim-3
Function:	May play a role in the development of immune responses and the development of Th1-mediated responses
Ligand/Receptor:	Putative ligand on resting CD4+ lymphocytes
Research Area:	Immunology, Inhibitory Molecules