Anti-Mouse CD3ε [500A2] In Vivo Antibody - Ultra Low Endotoxin
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- SKU:
- IVMB0151
- Product Type:
- In Vivo Monoclonal Antibody
- Clone:
- 500A2
- Protein:
- CD3-epsilon
- Isotype:
- Syrian Hamster IgG2a kappa
- Reactivity:
- Mouse
- Synonyms:
- T3
- Synonyms:
- CD3
- Research Area:
- T Cell Depletion
- Research Area:
- MHC & Immune Cell-Specific
- Endotoxin Level:
- Ultra Low Endotoxin
- Host Species:
- Syrian Hamster
- Applications:
- Blocking
- Applications:
- FC
- Applications:
- IHC FF
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Description
system_update_altTechnical Datasheet
| Product Name: | Anti-Mouse CD3ε [500A2] In Vivo Antibody - Ultra Low Endotoxin |
| Product Code: | IVMB0151 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | 500A2 |
| Protein: | CD3-epsilon |
| Product Type: | Monoclonal Antibody |
| Synonyms: | T3, CD3 |
| Isotype: | Syrian Hamster IgG2a κ |
| Reactivity: | Mouse |
| Immunogen: | Mouse T-cell receptor |
| Applications: | B, FC, IHC FF |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | ≤ 0.5 EU/mg as determined by the LAL method |
| Purity: | ≥98% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | B, FC, IHC FF |
| Recommended Usage: | FC The suggested concentration for this 500A2 antibody for staining cells in flow cytometry is ≤ 1.0 µg per 106 in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application. |
| Reactivity: | Mouse |
| Host Species: | Syrian Hamster |
| Specificity: | Clone 500A2 recognizes an epitope on mouse CD3-epsilon. |
| Antigen Distribution: | CD3-epsilon is primarily expressed on mature T cells and NK-T cells and at different levels on differentiation-dependent thymocytes. |
| Immunogen: | Mouse T-cell receptor |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | ≤ 0.5 EU/mg as determined by the LAL method |
| Purity: | ≥98% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition.
| Technical Datasheet: | View |
| Protein: | CD3-epsilon |
| Ligand/Receptor: | Peptide antigen/MHC-complex. |
| Research Area: | Cell Biology, Immunology, Signal Transduction |
Meet the team!
Shane Costigan
Territory Manager & Team Lead
Abdul Khadim
Sales Executive
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