Anti-Mouse CD3ε [145-2C11] In Vivo Antibody - Ultra Low Endotoxin

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SKU:
IVMB0059
Product Type:
In Vivo Monoclonal Antibody
Clone:
145-2C11
Protein:
CD3-epsilon
Isotype:
Armenian Hamster IgG
Reactivity:
Mouse
Synonyms:
CD3
Synonyms:
T3
Research Area:
T Cell Depletion
Endotoxin Level:
Ultra Low Endotoxin
Host Species:
Armenian Hamster
Applications:
Act
Applications:
Blocking
Applications:
CODEX
Applications:
CyTOF
Applications:
Depletion
Applications:
FA
Applications:
FC
Applications:
ICC
Applications:
IF
Applications:
IHC FF
Applications:
WB

Description

system_update_altTechnical Datasheet
Product Name: Anti-Mouse CD3ε [145-2C11] In Vivo Antibody - Ultra Low Endotoxin
Product Code:IVMB0059
Size:1mg, 5mg, 25mg, 50mg, 100mg
Clone:145-2C11
Protein:CD3-epsilon
Product Type:Monoclonal Antibody
Synonyms:CD3, T3
Isotype:Armenian Hamster IgG
Reactivity:Mouse
Immunogen:H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
Applications: Act, B, CyTOF®, Depletion, FA, FC, ICC, IF, IHC FF, PhenoCycler®, WB
Formulation: This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:≤ 0.5 EU/mg as determined by the LAL method
Purity: ≥98% monomer by analytical SEC
>95% by SDS Page
Preparation: Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling: Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Product CodeProduct NameEndotoxin Level
IVMB0058Anti-Mouse CD3-epsilon [145-2C11] In Vivo Antibody - Low EndotoxinLow
IVMB0059 Anti-Mouse CD3ε [145-2C11] In Vivo Antibody - Ultra Low Endotoxin Ultra Low
Applications: Act, B, CODEX®, CyTOF®, Depletion, FA, FC, ICC, IF, IHC FF, WB
Recommended Usage: FC
The suggested concentration for this 145-2C11 antibody for staining cells in flow cytometry is ≤ 1.0 µg per 106 in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application.
Reactivity:Mouse
Host Species:Armenian Hamster
Specificity:Clone 145-2C11 recognizes an epitope on mouse CD3-epsilon.
Antigen Distribution: CD3-epsilon is primarily expressed on mature T cells and NK-T cells and at different levels on differentiation-dependent thymocytes.
Immunogen:H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
Concentration:≥ 5.0 mg/ml
Endotoxin Level:≤ 0.5 EU/mg as determined by the LAL method
Purity: ≥98% monomer by analytical SEC
>95% by SDS Page
Formulation: This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation: Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling: Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition.

Technical Datasheet:View
Protein:CD3-epsilon
Ligand/Receptor:Peptide antigen/MHC-complex
Research Area:Immunology
Product Thumbnail
Armenian Hamster IgG Isotype Control
Clone PIP
Isotype Armenian Hamster IgG1
Endotoxin Level Ultra Low Endotoxin
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