Product Name:	Anti-Mouse CD3ε [145-2C11] In Vivo Antibody - Low Endotoxin
Product Code:	IVMB0058
Size:	1mg, 5mg, 25mg, 50mg, 100mg
Clone:	145-2C11
Protein:	CD3-epsilon
Product Type:	Monoclonal Antibody
Synonyms:	CD3, T3
Isotype:	Armenian Hamster IgG
Reactivity:	Mouse
Immunogen:	H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
Applications:	Act, B, CyTOF®, Depletion, FA, FC, ICC, IF, IHC FF, PhenoCycler®, WB
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC >95% by SDS Page
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Product Code	Product Name	Endotoxin Level
IVMB0058	Anti-Mouse CD3ε [145-2C11] In Vivo Antibody - Low Endotoxin	Low
IVMB0059	Anti-Mouse CD3-epsilon [145-2C11] In Vivo Antibody - Ultra Low Endotoxin	Ultra Low

Applications:	Act, B, CODEX®, CyTOF®, Depletion, FA, FC, ICC, IF, IHC FF, WB
Recommended Usage:	WB FC The suggested concentration for this 145-2C11 antibody for staining cells in flow cytometry is ≤ 1.0 µg per 10⁶ in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application.
Reactivity:	Mouse

Host Species:	Armenian Hamster
Specificity:	Clone 145-2C11 recognizes an epitope on mouse CD3-epsilon.
Antigen Distribution:	CD3-epsilon is primarily expressed on mature T cells and NK-T cells and at different levels on differentiation-dependent thymocytes.
Immunogen:	H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
Concentration:	≥ 5.0 mg/ml
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC >95% by SDS Page
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition.

Technical Datasheet:	View
Protein:	CD3-epsilon
Function:	TCR signal transduction, T cell activation, antigen recognition
Ligand/Receptor:	Peptide antigen/MHC-complex
Research Area:	Immunology