Product Name:	Anti-Mouse CD172a In Vivo Antibody - Low Endotoxin
Product Code:	IVMB0029
Size:	1mg, 5mg, 25mg, 50mg, 100mg
Clone:	P84
Protein:	CD172a
Product Type:	Monoclonal Antibody
Synonyms:	SIRPα, SHPS-1, BIT, P84, PTPNS1, CD172 antigen-like family member A
Isotype:	Rat IgG1 κ
Reactivity:	Mouse
Immunogen:	Mouse brain membrane protein
Applications:	B, CyTOF®, FC, IHC FF, IP
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC >95% by SDS Page
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Product Code	Product Name	Endotoxin Level
IVMB0029	Anti-Mouse CD172a In Vivo Antibody - Low Endotoxin	Low
IVMB0030	Anti-Mouse CD172a In Vivo Antibody - Ultra Low Endotoxin	Ultra Low

Applications:	B, CyTOF®, FC, IHC FF, IP
Recommended Usage:	FC The suggested concentration for this P84 antibody for staining cells in flow cytometry is ≤ 1.0 µg per 10⁶ in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application. CyTOF®
Reactivity:	Mouse

Host Species:	Rat
Specificity:	Clone P84 recognizes an epitope on mouse CD172a.
Antigen Distribution:	CD172a is expressed on monocytes, macrophages, dendritic cells, and neuronal cells.
Immunogen:	Mouse brain membrane protein
Concentration:	≥ 5.0 mg/ml
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC >95% by SDS Page
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

CD172a antibody, clone P84, recognizes CD172a, also known as single regulatory protein α (SIRPα) (signal regulatory protein alpha) or Src homology 2 domain-containing phosphatase substrate-1 (SHP-1), a type I transmembrane glycoprotein with three Ig-like extracellular domains and two cytoplasmic immunoreceptor tyrosine-based inhibition motifs (ITIMs)1. SIRPα is expressed predominantly in myeloid cells2 - including monocytes, macrophages, and dendritic cells (DCs) - and neuronal cells3. The extracellular ligand for SIRPα, CD47 (or integrin-associated protein [IAP])4, is expressed in most cell types5. In macrophages, ligation of SIRPα by CD47 inhibits macrophage phagocytosis of self cells6,7. SIRPα also negatively regulates DC-mediated T cell activation and DC maturation8-10. CD47 is also upregulated on tumor cells, inhibiting the phagocytosis of tumor cells by macrophages11. Therapeutics targeting the CD47-SIRPα interaction, including antibodies and fusion proteins, are currently under preclinical and clinical study for various malignancies as a monotherapy or in combination with other therapeutics12.

Technical Datasheet:	View
Protein:	CD172a
Function:	Negative regulation of several biological processes
Ligand/Receptor:	CD47, SP-A, SP-D
Research Area:	Cell Adhesion, Innate Immunity, Neuroscience