Cell Cycle Antibodies 1

Anti-MonoMethyl-UHRF1-K385 Antibody (CAB16008)

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SKU:
CAB16008
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-MonoMethyl-UHRF1-K385 Antibody
Antibody SKU:CAB16008
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF DB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic monomethylated peptide around K385 of human UHRF1 (NP_001041666.1).
Application:WB IHC IF DB
Recommended Dilution:DB 1:500 - 1:2000 WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:C6
Immunogen:A synthetic monomethylated peptide around K385 of human UHRF1 (NP_001041666.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:KAKM A
Gene ID:29128
Uniprot:Q96T88
Cellular Location:Nucleus
Calculated MW:89kDa/91kDa
Observed MW:91kDa
Synonyms:UHRF1, ICBP90, Np95, RNF106, TDRD22, hNP95, hUHRF1, huNp95
Background:This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. It is regarded as a hub protein for the integration of epigenetic information. This gene is up-regulated in various cancers, and it is therefore considered to be a therapeutic target. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene exists on chromosome 12.
UniProt Protein Function:UHRF1: Multidomain protein that acts as a key epigenetic regulator by bridging DNA methylation and chromatin modification. Specifically recognizes and binds hemimethylated DNA at replication forks via its YDG domain and recruits DNMT1 methyltransferase to ensure faithful propagation of the DNA methylation patterns through DNA replication. In addition to its role in maintenance of DNA methylation, also plays a key role in chromatin modification: through its tudor-like regions and PHD- type zinc fingers, specifically recognizes and binds histone H3 trimethylated at 'Lys-9' (H3K9me3) and unmethylated at 'Arg-2' (H3R2me0), respectively, and recruits chromatin proteins. Enriched in pericentric heterochromatin where it recruits different chromatin modifiers required for this chromatin replication. Also localizes to euchromatic regions where it negatively regulates transcription possibly by impacting DNA methylation and histone modifications. Has E3 ubiquitin-protein ligase activity by mediating the ubiquitination of target proteins such as histone H3 and PML. It is still unclear how E3 ubiquitin-protein ligase activity is related to its role in chromatin in vivo. May be involved in DNA repair. Defects in UHRF1 may be a cause of cancers. Overexpressed in many different forms of human cancers, including bladder, breast, cervical, colorectal and prostate cancers, as well as pancreatic adenocarcinomas, rhabdomyosarcomas and gliomas. Plays an important role in the correlation of histone modification and gene silencing in cancer progression. Expression is associated with a poor prognosis in patients with various cancers, suggesting that it participates in cancer progression. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:DNA-binding; Ligase; Ubiquitin conjugating system; Ubiquitin ligase; EC 6.3.2.-; EC 6.3.2.19; Transcription factor

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: euchromatin; heterochromatin; nuclear chromatin; nuclear heterochromatin; nuclear matrix; nucleus; replication fork

Molecular Function:histone binding; identical protein binding; methyl-CpG binding; methylated histone residue binding; nucleosomal histone binding; protein binding; transcription factor activity; ubiquitin-protein ligase activity; zinc ion binding

Biological Process: cell proliferation; histone monoubiquitination; histone ubiquitination; maintenance of DNA methylation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cellular protein metabolic process; positive regulation of transcription from RNA polymerase II promoter; protein autoubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process

NCBI Summary:This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. It is regarded as a hub protein for the integration of epigenetic information. This gene is up-regulated in various cancers, and it is therefore considered to be a therapeutic target. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene exists on chromosome 12. [provided by RefSeq, Feb 2014]
UniProt Code:Q96T88
NCBI GenInfo Identifier:67462077
NCBI Gene ID:29128
NCBI Accession:Q96T88.1
UniProt Secondary Accession:Q96T88,Q2HIX7, Q8J022, Q9H6S6, Q9P115, Q9P1U7, A0JBR2 A8K024, B2RBA9,
UniProt Related Accession:Q96T88
Molecular Weight:91,116 Da
NCBI Full Name:E3 ubiquitin-protein ligase UHRF1
NCBI Synonym Full Names:ubiquitin like with PHD and ring finger domains 1
NCBI Official Symbol:UHRF1  
NCBI Official Synonym Symbols:Np95; hNP95; ICBP90; RNF106; TDRD22; hUHRF1; huNp95  
NCBI Protein Information:E3 ubiquitin-protein ligase UHRF1
UniProt Protein Name:E3 ubiquitin-protein ligase UHRF1
UniProt Synonym Protein Names:Inverted CCAAT box-binding protein of 90 kDa; Nuclear protein 95; Nuclear zinc finger protein Np95; HuNp95; hNp95; RING finger protein 106; Transcription factor ICBP90; Ubiquitin-like PHD and RING finger domain-containing protein 1; hUHRF1; Ubiquitin-like-containing PHD and RING finger domains protein 1
Protein Family:UHRF1-binding protein
UniProt Gene Name:UHRF1  
UniProt Entry Name:UHRF1_HUMAN
Anti-MonoMethyl-UHRF1-K385 Antibody (CAB16008)Western blot analysis of extracts of C6 cells, using MonoMethyl-UHRF1-K385 antibody (CAB16008) at 1:1000 dilution.C6 cells were treated by MG132(50 uM) at 37'C for 90 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-MonoMethyl-UHRF1-K385 Antibody (CAB16008)Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-UHRF1-K385 antibody (CAB16008) at 1:1000 dilution.
Anti-MonoMethyl-UHRF1-K385 Antibody (CAB16008)Immunofluorescence analysis of U2OS cells using MonoMethyl-UHRF1-K385 antibody (CAB16008) at dilution of 1:100. Blue: DAPI for nuclear staining.
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