Antibody Name:	Anti-MMRN1 Antibody
Antibody SKU:	CAB6658
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 949-1228 of human MMRN1 (NP_031377.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	HepG2, U-251MG, Mouse lung, Mouse spleen

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 949-1228 of human MMRN1 (NP_031377.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	IKHS LPDI QLLQ KGLT EFVE PIIQ IKTQ AALS NLTC CIDR SLPG SLAN VVKS QKQV KSLP KKIN ALKK PTVN LTTV LIGR TQRN TDNI IYPE EYSS CSRH PCQN GGTC INGR TSFT CACR HPFT GDNC TIKL VEEN ALAP DFSK GSYR YAPM VAFF ASHT YGMT IPGP ILFN NLDV NYGA SYTP RTGK FRIP YLGV YVFK YTIE SFSA HISG FLVV DGID KLAF ESEN INSE IHCD RVLT GDAL LELN YGQE VWLR LAKG TIPA KFPP VTTF SGYL LYRT
Gene ID:	22915
Uniprot:	Q13201
Cellular Location:	Secreted
Calculated MW:	58kDa/138kDa
Observed MW:	180kDa

Synonyms:

MMRN1, ECM, EMILIN4, GPIa*, MMRN

Background:

Multimerin is a massive, soluble protein found in platelets and in the endothelium of blood vessels. It is comprised of subunits linked by interchain disulfide bonds to form large, variably sized homomultimers. Multimerin is a factor V/Va-binding protein and may function as a carrier protein for platelet factor V. It may also have functions as an extracellular matrix or adhesive protein. Recently, patients with an unusual autosomal-dominant bleeding disorder (factor V Quebec) were found to have a deficiency of platelet multimerin.

UniProt Protein Function:	MMRN1: Carrier protein for platelet (but not plasma) factor V/Va. Plays a role in the storage and stabilization of factor V in platelets. Upon release following platelet activation, may limit platelet and plasma factor Va-dependent thrombin generation. Ligand for integrin alpha-IIb/beta-3 and integrin alpha-V/beta-3 on activated platelets, and may function as an extracellular matrix or adhesive protein. Deficiency in multimerin-1 due to proteolytic degradation within the platelet alpha granules is associated with an autosomal dominant bleeding disorder (factor V Quebec). 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 4q22 Cellular Component: extracellular region Molecular Function:protein binding Biological Process: cell adhesion; platelet degranulation
NCBI Summary:	Multimerin is a massive, soluble protein found in platelets and in the endothelium of blood vessels. It is comprised of subunits linked by interchain disulfide bonds to form large, variably sized homomultimers. Multimerin is a factor V/Va-binding protein and may function as a carrier protein for platelet factor V. It may also have functions as an extracellular matrix or adhesive protein. Recently, patients with an unusual autosomal-dominant bleeding disorder (factor V Quebec) were found to have a deficiency of platelet multimerin. [provided by RefSeq, Jul 2008]
UniProt Code:	Q13201
NCBI GenInfo Identifier:	143811421
NCBI Gene ID:	22915
NCBI Accession:	Q13201.3
UniProt Secondary Accession:	Q13201,Q4W5L1, Q6P3T8, Q6ZUL9,
UniProt Related Accession:	Q13201
Molecular Weight:	58,170 Da
NCBI Full Name:	Multimerin-1
NCBI Synonym Full Names:	multimerin 1
NCBI Official Symbol:	MMRN1
NCBI Official Synonym Symbols:	ECM; MMRN; GPIa*; EMILIN4
NCBI Protein Information:	multimerin-1
UniProt Protein Name:	Multimerin-1
UniProt Synonym Protein Names:	EMILIN-4; Elastin microfibril interface located protein 4; Elastin microfibril interfacer 4
Protein Family:	Multimerin
UniProt Gene Name:	MMRN1
UniProt Entry Name:	MMRN1_HUMAN

	Western blot analysis of extracts of various cell lines, using MMRN1 Antibody (CAB6658) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded human lung using MMRN1 antibody (CAB6658) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using MMRN1 antibody (CAB6658). Blue: DAPI for nuclear staining.