Cell Biology Antibodies 7
Anti-MMP12 Antibody (CAB1709)
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- SKU:
- CAB1709
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-MMP12 Antibody |
| Antibody SKU: | CAB1709 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide of human MMP12 |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:1000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Human heart |
| Immunogen: | A synthetic peptide of human MMP12 |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 4321 |
| Uniprot: | P39900 |
| Cellular Location: | Secreted, extracellular matrix, extracellular space |
| Calculated MW: | 54kDa |
| Observed MW: | 51kDa |
| Synonyms: | MMP12, HME, ME, MME, MMP-12 |
| Background: | This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease degrades soluble and insoluble elastin. This gene may play a role in aneurysm formation and mutations in this gene are associated with lung function and chronic obstructive pulmonary disease (COPD). This gene is part of a cluster of MMP genes on chromosome 11. |
| UniProt Protein Function: | MMP12: May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3. Belongs to the peptidase M10A family. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide; EC 3.4.24.65; Protease Chromosomal Location of Human Ortholog: 11q22.3 Cellular Component: proteinaceous extracellular matrix; extracellular region Molecular Function:zinc ion binding; metalloendopeptidase activity; endopeptidase activity; calcium ion binding Biological Process: collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; positive regulation of epithelial cell proliferation involved in wound healing; proteolysis; wound healing, spreading of epidermal cells |
| NCBI Summary: | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P39900 |
| NCBI GenInfo Identifier: | 729179 |
| NCBI Gene ID: | 4321 |
| NCBI Accession: | P39900.1 |
| UniProt Secondary Accession: | P39900,Q2M1L9, B2R9X8, B7ZLF6, |
| UniProt Related Accession: | P39900 |
| Molecular Weight: | |
| NCBI Full Name: | Macrophage metalloelastase |
| NCBI Synonym Full Names: | matrix metallopeptidase 12 (macrophage elastase) |
| NCBI Official Symbol: | MMP12 |
| NCBI Official Synonym Symbols: | ME; HME; MME; MMP-12 |
| NCBI Protein Information: | macrophage metalloelastase; matrix metalloproteinase 12 (macrophage elastase) |
| UniProt Protein Name: | Macrophage metalloelastase |
| UniProt Synonym Protein Names: | Macrophage elastase; ME; hME; Matrix metalloproteinase-12; MMP-12 |
| Protein Family: | Macrophage metalloelastase |
| UniProt Gene Name: | MMP12 |
| UniProt Entry Name: | MMP12_HUMAN |
 | Western blot analysis of extracts of human heart, using MMP12 antibody (CAB1709). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
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