Name: Anti-MLST8 Antibody (CAB1059)[KO Validated]
Brand: KO Validated Antibodies 1
SKU: CAB1059
Availability: OutOfStock

Antibody Name:	Anti-MLST8 Antibody [KO Validated]
Antibody SKU:	CAB1059
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-326 of human MLST8 (NP_001186103.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, HeLa, K-562, 22Rv1, Mouse brain, Mouse kidney, Mouse thymus, Rat brain, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-326 of human MLST8 (NP_001186103.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MNTS PGTV GSDP VILA TAGY DHTV RFWQ AHSG ICTR TVQH QDSQ VNAL EVTP DRSM IAAA GYQH IRMY DLNS NNPN PIIS YDGV NKNI ASVG FHED GRWM YTGG EDCT ARIW DLRS RNLQ CQRI FQVN APIN CVCL HPNQ AELI VGDQ SGAI HIWD LKTD HNEQ LIPE PEVS ITSA HIDP DASY MAAV NSTG NCYV WNLT GGIG DEVT QLIP KTKI PAHT RYAL QCRF SPDS TLLA TCSA DQTC KIWR TSNF SLMT ELSI KSGN PGES SRGW MWGC AFSG DSQY IVTA SSDN LARL WCVE TGEI KREY GGHQ KAVV CLAF NDSV LG
Gene ID:	64223
Uniprot:	Q9BVC4
Cellular Location:	Cytoplasm
Calculated MW:	23kDa/28kDa/35kDa
Observed MW:	37kDa

Synonyms:	MLST8, GBL, GbetaL, LST8, POP3, WAT1
Background:

UniProt Protein Function:	LST8: Subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1- mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, LST8 interacts directly with MTOR and enhances its kinase activity. In nutrient- poor conditions, stabilizes the MTOR-RPTOR interaction and favors RPTOR-mediated inhibition of MTOR activity. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum- induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains MTOR, MLST8, RPTOR, AKT1S1/PRAS40 and DEPTOR. mTORC1 binds to and is inhibited by FKBP12-rapamycin. Part of the mammalian target of rapamycin complex 2 (mTORC2) which contains MTOR, MLST8, PRR5, RICTOR, MAPKAP1 and DEPTOR. Contrary to mTORC1, mTORC2 does not bind to and is not sensitive to FKBP12- rapamycin. Interacts directly with MTOR and RPTOR. Interacts with RHEB. Broadly expressed, with highest levels in skeletal muscle, heart and kidney. Belongs to the WD repeat LST8 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Autophagy Chromosomal Location of Human Ortholog: 16p13.3 Cellular Component: nucleoplasm; cytoplasm; cytosol Molecular Function:protein binding Biological Process: epidermal growth factor receptor signaling pathway; positive regulation of peptidyl-tyrosine phosphorylation; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; positive regulation of actin filament polymerization; nerve growth factor receptor signaling pathway; regulation of actin cytoskeleton organization and biogenesis; T cell costimulation; insulin receptor signaling pathway; innate immune response; vascular endothelial growth factor receptor signaling pathway; positive regulation of TOR signaling pathway
UniProt Code:	Q9BVC4
NCBI GenInfo Identifier:	74761285
NCBI Gene ID:	64223
NCBI Accession:	Q9BVC4.1
UniProt Related Accession:	Q9BVC4
Molecular Weight:
NCBI Full Name:	Target of rapamycin complex subunit LST8
NCBI Synonym Full Names:	MTOR associated protein, LST8 homolog
NCBI Official Symbol:	MLST8
NCBI Official Synonym Symbols:	GBL; LST8; POP3; WAT1; GbetaL
NCBI Protein Information:	target of rapamycin complex subunit LST8
UniProt Protein Name:	Target of rapamycin complex subunit LST8
UniProt Synonym Protein Names:	G protein beta subunit-like; Gable; Protein GbetaL; Mammalian lethal with SEC13 protein 8; mLST8
UniProt Gene Name:	MLST8
UniProt Entry Name:	LST8_HUMAN

	Western blot analysis of extracts of various cell lines, using MLST8 antibody (CAB1059) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Western blot analysis of extracts from normal (control) and MLST8 knockout (KO) HeLa cells, using MLST8 antibody (CAB1059) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.