Name: Anti-MLC1 Antibody (CAB12840)
Brand: Signal Transduction Antibodies 1
SKU: CAB12840
Availability: OutOfStock

Antibody Name:	Anti-MLC1 Antibody
Antibody SKU:	CAB12840
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human MLC1 (NP_055981.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	SH-SY5Y

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human MLC1 (NP_055981.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SYDV LLLL LLLV LLLQ AGLN TGTA IQCV RFKV SARL QGAS WDTQ NGPQ ERLA GEVA RSPL KEFD KEKA WRAV VVQM AQ
Gene ID:	23209
Uniprot:	Q15049
Cellular Location:	Cell membrane, Cytoplasm, Endoplasmic reticulum, Membrane, Multi-pass membrane protein, perinuclear region
Calculated MW:	37kDa/41kDa
Observed MW:	45kDa

Synonyms:	MLC1, LVM, MLC, VL
Background:	The function of this gene product is unknown; however, homology to other proteins suggests that it may be an integral membrane transporter. Mutations in this gene have been associated with megalencephalic leukoencephalopathy with subcortical cysts, an autosomal recessive neurological disorder. Alternatively spliced transcript variants encoding different isoforms have been identified.

UniProt Protein Function:	MLC1: Regulates the response of astrocytes to hypo-osmosis by promoting calcium influx. Defects in MLC1 are a cause of leukoencephalopathy megalencephalic with subcortical cysts type 1 (MLC1). MLC1 is a syndrome of cerebral leukoencephalopathy and megalencephaly characterized by ataxia, spasticity, seizures, delay in motor development and mild mental retardation. The brain appears swollen on magnetic resonance imaging, with diffuse white- matter abnormalities and the invariable presence of subcortical cysts in frontal and temporal lobes.
UniProt Protein Details:	Protein type:Cell surface; Membrane protein, integral; Membrane protein, multi-pass; Transporter Chromosomal Location of Human Ortholog: 22q13.33 Cellular Component: basolateral plasma membrane; caveola; clathrin-coated vesicle; cytoplasm; cytoplasmic vesicle; early endosome; endoplasmic reticulum; endosome; integral to membrane; lipid raft; lysosome; perinuclear region of cytoplasm; plasma membrane; recycling endosome Molecular Function:protein binding; protein complex binding; protein transporter activity Biological Process: positive regulation of intracellular transport; protein oligomerization; regulation of response to osmotic stress; vesicle-mediated transport Disease: Megalencephalic Leukoencephalopathy With Subcortical Cysts 1
NCBI Summary:	The function of this gene product is unknown; however, homology to other proteins suggests that it may be an integral membrane transporter. Mutations in this gene have been associated with megalencephalic leukoencephalopathy with subcortical cysts, an autosomal recessive neurological disorder. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
UniProt Code:	Q15049
NCBI GenInfo Identifier:	20141590
NCBI Gene ID:	23209
NCBI Accession:	Q15049.5
UniProt Secondary Accession:	Q15049,Q5JZ83, Q8TAG4, Q96RP5, Q9UGY8, B3KW61, B7Z659
UniProt Related Accession:	Q15049
Molecular Weight:	41kDa
NCBI Full Name:	Membrane protein MLC1
NCBI Synonym Full Names:	megalencephalic leukoencephalopathy with subcortical cysts 1
NCBI Official Symbol:	MLC1
NCBI Official Synonym Symbols:	VL; LVM; MLC
NCBI Protein Information:	membrane protein MLC1
UniProt Protein Name:	Membrane protein MLC1
Protein Family:	Myosin
UniProt Gene Name:	MLC1

Western blot analysis of extracts of SH-SY5Y cells, using MLC1 antibody (CAB12840) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Antibodies

MLC1 Antibody (PACO10506)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)