Developmental Biology
Anti-MIXL1 Antibody (CAB17223)
- SKU:
- CAB17223
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Description
| Antibody Name: | Anti-MIXL1 Antibody |
| Antibody SKU: | CAB17223 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide of human MIXL1. |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: |
| Immunogen: | A synthetic peptide of human MIXL1. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 83881 |
| Uniprot: | Q9H2W2 |
| Cellular Location: | |
| Calculated MW: | |
| Observed MW: | Refer to figures |
| Synonyms: | MIXL1, MILD1, MIX, MIXL |
| Background: | Homeodomain proteins, such as MIXL1, are transcription factors that regulate cell fate during development (Hart et al., 2005 [PubMed 15982639]). |
| UniProt Protein Function: | MIXL1: Transcription factor that play a central role in proper axial mesendoderm morphogenesis and endoderm formation. Required for efficient differentiation of cells from the primitive streak stage to blood, by acting early in the recruitment and/or expansion of mesodermal progenitors to the hemangioblastic and hematopoietic lineages. Also involved in the morphogenesis of the heart and the gut during embryogenesis. Acts as a negative regulator of brachyury expression. Belongs to the paired homeobox family. |
| UniProt Protein Details: | Protein type:DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 1q42.12 Cellular Component: nuclear chromatin Molecular Function:protein homodimerization activity Biological Process: endoderm development; gastrulation; gut development; heart development; hemopoietic progenitor cell differentiation; positive regulation of transcription from RNA polymerase II promoter |
| NCBI Summary: | Homeodomain proteins, such as MIXL1, are transcription factors that regulate cell fate during development (Hart et al., 2005 [PubMed 15982639]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q9H2W2 |
| NCBI GenInfo Identifier: | 74762734 |
| NCBI Gene ID: | 83881 |
| NCBI Accession: | Q9H2W2.1 |
| UniProt Secondary Accession: | Q9H2W2,B7ZLF9, |
| UniProt Related Accession: | Q9H2W2 |
| Molecular Weight: | 25,605 Da |
| NCBI Full Name: | Homeobox protein MIXL1 |
| NCBI Synonym Full Names: | Mix paired-like homeobox |
| NCBI Official Symbol: | MIXL1 |
| NCBI Official Synonym Symbols: | MIX; MIXL; MILD1 |
| NCBI Protein Information: | homeobox protein MIXL1 |
| UniProt Protein Name: | Homeobox protein MIXL1 |
| UniProt Synonym Protein Names: | Homeodomain protein MIX; hMix; MIX1 homeobox-like protein 1; Mix.1 homeobox-like protein |
| UniProt Gene Name: | MIXL1 |
| UniProt Entry Name: | MIXL1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MIXL1 antibody (CAB17223) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5min. |
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