Signal Transduction Antibodies 3

Anti-MIB1 Antibody (CAB8588)

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SKU:
CAB8588
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-MIB1 Antibody
Antibody SKU:CAB8588
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 560-860 of human MIB1 (NP_065825.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, A-549, HepG2, Mouse stomach, Mouse lung, Mouse kidney , Rat testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 560-860 of human MIB1 (NP_065825.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:DSEG DTPL HDAI SKKR DDIL AVLL EAGA DVTI TNNN GFNA LHHA ALRG NPSA MRVL LSKL PRPW IVDE KKDD GYTA LHLA ALNN HVEV AELL VHQG NANL DIQN VNQQ TALH LAVE RQHT QIVR LLVR AGAK LDIQ DKDG DTPL HEAL RHHT LSQL RQLQ DMQD VGKV DAAW EPSK NTLI MGLG TQGA EKKS AASI ACFL AANG ADLS IRNK KGQS PLDL CPDP NLCK ALAK CHKE KVSG QVGS RSPS MISN DSET LEEC MVCS DMKR DTLF GPCG HIAT CSLC SPRV KKCL ICKE QVQS R
Gene ID:57534
Uniprot:Q86YT6
Cellular Location:Cell membrane, Cytoplasm, centriolar satellite, centrosome, cytoskeleton, microtubule organizing center
Calculated MW:110kDa
Observed MW:110kDa
Synonyms:MIB1, DIP-1, DIP1, LVNC7, MIB, ZZANK2, ZZZ6
Background:This gene encodes a protein containing multiple ankyrin repeats and RING finger domains that functions as an E3 ubiquitin ligase. The encoded protein positively regulates Notch signaling by ubiquitinating the Notch receptors, thereby facilitating their endocytosis. This protein may also promote the ubiquitination and degradation of death-associated protein kinase 1 (DAPK1).
UniProt Protein Function:MIB1: E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF- induced apoptosis. Mediates 'Lys-63'-linked polyubiquitination of TBK1, which probably participates in kinase activation. Widely expressed at low level. Expressed at higher level in spinal cord, ovary, whole brain, and all specific brain regions examined.
UniProt Protein Details:

Protein type:Ubiquitin conjugating system; EC 6.3.2.-; EC 6.3.2.19; Ubiquitin ligase; Ligase

Chromosomal Location of Human Ortholog: 18q11.2

Cellular Component: centrosome; postsynaptic density; cytoplasm; plasma membrane; cytoplasmic vesicle; cytosol

Molecular Function:protein binding; zinc ion binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: blood vessel development; somitogenesis; negative regulation of neuron differentiation; Notch signaling pathway; in utero embryonic development; protein ubiquitination; positive regulation of endocytosis; endocytosis; heart looping; neural tube formation

Disease: Left Ventricular Noncompaction 7

NCBI Summary:This gene encodes a protein containing multiple ankyrin repeats and RING finger domains that functions as an E3 ubiquitin ligase. The encoded protein positively regulates Notch signaling by ubiquitinating the Notch receptors, thereby facilitating their endocytosis. This protein may also promote the ubiquitination and degradation of death-associated protein kinase 1 (DAPK1). [provided by RefSeq, Jun 2013]
UniProt Code:Q86YT6
NCBI GenInfo Identifier:30348954
NCBI Gene ID:57534
NCBI Accession:NP_065825
UniProt Secondary Accession:Q86YT6,Q2TB37, Q68D01, Q6YI51, Q8NBY0, Q8TCB5, Q8TCL7 Q9P2M3, B0YJ38,
UniProt Related Accession:Q86YT6
Molecular Weight:Predicted: 89; 102; 111 kDaObserved: 103 kDa
NCBI Full Name:E3 ubiquitin-protein ligase MIB1
NCBI Synonym Full Names:mindbomb E3 ubiquitin protein ligase 1
NCBI Official Symbol:MIB1  
NCBI Official Synonym Symbols:MIB; DIP1; ZZZ6; DIP-1; LVNC7; ZZANK2  
NCBI Protein Information:E3 ubiquitin-protein ligase MIB1; DAPK-interacting protein 1; ubiquitin ligase mind bomb; zinc finger ZZ type with ankyrin repeat domain protein 2
UniProt Protein Name:E3 ubiquitin-protein ligase MIB1
UniProt Synonym Protein Names:DAPK-interacting protein 1; DIP-1; Mind bomb homolog 1; Zinc finger ZZ type with ankyrin repeat domain protein 2
Protein Family:E3 ubiquitin-protein ligase
UniProt Gene Name:MIB1  
UniProt Entry Name:MIB1_HUMAN
Anti-MIB1 Polyclonal Antibody (CAB8588)Western blot analysis of extracts of various cell lines, using MIB1 antibody (CAB8588) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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