Antibody Name:	Anti-MFI2 Antibody
Antibody SKU:	CAB6653
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-200 of human MFI2 (NP_201573.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, SW480, A375, Mouse liver, Mouse brain, Mouse thymus

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-200 of human MFI2 (NP_201573.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GMEV RWCA TSDP EQHK CGNM SEAF REAG IQPS LLCV RGTS ADHC VQLI AAQE ADAI TLDG GAIY EAGK EHGL KPVV GEVY DQEV GTSY YAVA VVRR SSHV TIDT LKGV KSCH TGIN RTVG WNVP VGYL VESG RLSV MGCD VLKA VSDY FGGS CVPG AGET SYSE SLCR LCRG DSSG EGVC D
Gene ID:	4241
Uniprot:	P08582
Cellular Location:	Cell membrane, GPI-anchor, Lipid-anchor
Calculated MW:	32kDa/80kDa
Observed MW:	110kDa

Synonyms:

MELTF, CD228, MAP97, MFI2, MTF1, MTf

Background:

The protein encoded by this gene is a cell-surface glycoprotein found on melanoma cells. The protein shares sequence similarity and iron-binding properties with members of the transferrin superfamily. The importance of the iron binding function has not yet been identified. This gene resides in the same region of chromosome 3 as members of the transferrin superfamily. Alternative splicing results in two transcript variants.

UniProt Protein Function:	MFI2: Involved in iron cellular uptake. Seems to be internalized and then recycled back to the cell membrane. Binds a single atom of iron per subunit. Could also bind zinc. Belongs to the transferrin family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, GPI anchor Chromosomal Location of Human Ortholog: 3q28-q29 Cellular Component: cell surface; anchored to plasma membrane; integral to plasma membrane Molecular Function:protein binding; ferric iron binding; iron ion binding Biological Process: cellular iron ion homeostasis; regulation of cell growth; regulation of cell proliferation
NCBI Summary:	The protein encoded by this gene is a cell-surface glycoprotein found on melanoma cells. The protein shares sequence similarity and iron-binding properties with members of the transferrin superfamily. The importance of the iron binding function has not yet been identified. This gene resides in the same region of chromosome 3 as members of the transferrin superfamily. Alternative splicing results in two transcript variants. [provided by RefSeq, Jul 2008]
UniProt Code:	P08582
NCBI GenInfo Identifier:	338817914
NCBI Gene ID:	4241
NCBI Accession:	P08582.2
UniProt Secondary Accession:	P08582,Q9BQE2,
UniProt Related Accession:	P08582
Molecular Weight:	738
NCBI Full Name:	Melanotransferrin
NCBI Synonym Full Names:	antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5
NCBI Official Symbol:	MFI2
NCBI Official Synonym Symbols:	MTF1; CD228; MAP97
NCBI Protein Information:	melanotransferrin; membrane-bound transferrin-like protein; melanoma-associated antigen p97, isoform 2
UniProt Protein Name:	Melanotransferrin
UniProt Synonym Protein Names:	Melanoma-associated antigen p97; CD_antigen: CD228
UniProt Gene Name:	MFI2
UniProt Entry Name:	TRFM_HUMAN

	Western blot analysis of extracts of various cell lines, using MFI2 antibody (CAB6653) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of L929 cells using MFI2 Rabbit pAb (CAB6653) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.