Cell Biology Antibodies 12
Anti-METTL3 Antibody (CAB8370)
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- SKU:
- CAB8370
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IHC
- Applications:
- IF
- Applications:
- IP
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-METTL3 Antibody |
| Antibody SKU: | CAB8370 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-156 of human METTL3 (NP_062826.2). |
| Application: | WB IHC IF IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:20 - 1:50 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2, Mouse spleen, Mouse thymus |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-156 of human METTL3 (NP_062826.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSDT WSSI QAHK KQLD SLRE RLQR RRKQ DSGH LDLR NPEA ALSP TFRS DSPV PTAP TSGG PKPS TASA VPEL ATDP ELEK KLLH HLSD LALT LPTD AVSI CLAI STPD APAT QDGV ESLL QKFA AQEL IEVK RGLL QDDA HPTL VTYA DHSK LSAM |
| Gene ID: | 56339 |
| Uniprot: | Q86U44 |
| Cellular Location: | Nucleus speckle |
| Calculated MW: | 25kDa/64kDa |
| Observed MW: | 80kDa |
| Synonyms: | IME4, M6A, MT-A70, Spo8, hMETTL3, METTL3 |
| Background: | This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine. |
| UniProt Protein Function: | METTL3: N6-methyltransferase that methylates adenosine residues of some mRNAs. N6-methyladenosine (m6A), which is present at internal sites of some mRNAs, may play a role in the efficiency of mRNA splicing, transport or translation. Belongs to the MT-A70-like family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Methyltransferase; EC 2.1.1.62; RNA processing Chromosomal Location of Human Ortholog: 14q11.1 Cellular Component: nucleoplasm; nuclear speck; nucleus Molecular Function:mRNA (2'-O-methyladenosine-N6-)-methyltransferase activity; RNA binding Biological Process: circadian rhythm; stem cell maintenance; RNA methylation; gene expression; mRNA processing |
| NCBI Summary: | This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q86U44 |
| NCBI GenInfo Identifier: | 21361827 |
| NCBI Gene ID: | 56339 |
| NCBI Accession: | NP_062826.2 |
| UniProt Secondary Accession: | Q86U44,O14736, Q86V05, Q9HB32, |
| UniProt Related Accession: | Q86U44 |
| Molecular Weight: | 25,479 Da |
| NCBI Full Name: | N6-adenosine-methyltransferase 70 kDa subunit |
| NCBI Synonym Full Names: | methyltransferase like 3 |
| NCBI Official Symbol: | METTL3 |
| NCBI Official Synonym Symbols: | M6A; IME4; Spo8; MT-A70 |
| NCBI Protein Information: | N6-adenosine-methyltransferase 70 kDa subunit; mRNA m(6)A methyltransferase; methyltransferase-like protein 3; adoMet-binding subunit of the human mRNA (N6-adenosine)-methyltransferase |
| UniProt Protein Name: | N6-adenosine-methyltransferase 70 kDa subunit |
| UniProt Synonym Protein Names: | Methyltransferase-like protein 3 |
| Protein Family: | N6-adenosine-methyltransferase |
| UniProt Gene Name: | METTL3 |
| UniProt Entry Name: | MTA70_HUMAN |
 | Western blot analysis of extracts of various cell lines, using METTL3 antibody (CAB8370) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 1s. |
 | Immunohistochemistry of paraffin-embedded human esophageal cancer using METTL3 Rabbit pAb (CAB8370) at dilution of 1:100 (40x lens). |
 | Confocal immunofluorescence analysis of Hela cells using Anti-METTL3 Polyclonal Antibody (CAB8370) at dilution of 1:200. Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 150ug extracts of 293T cells using 3ug METTL3 antibody (CAB8370). Western blot was performed from the immunoprecipitate using METTL3 antibody (CAB8370) at a dilition of 1:1000. |
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