KO Validated Antibodies 2

[KO Validated] MEK2 Rabbit Monoclonal Antibody (CAB19078)

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SKU:
CAB19078
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
Antibody Type:
Monoclonal Antibody
Reactivity:
Human, Mouse
Synonyms:
CFC4, MEK2, MKK2, MAPKK2, PRKMK2, [KO/KD Validated] MEK2
Research Use:
Cell Biology
Frequently bought together:

Description

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[KO Validated] MEK2 Monoclonal Antibody (CAB19078)

The [KO Validated] MEK2 Monoclonal Antibody (CAB19078) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity in detecting MEK2 in human samples, making it an ideal choice for Western blot applications. By targeting the MEK2 protein, this antibody allows for accurate and reliable analysis of signaling pathways involved in cell growth, differentiation, and survival.MEK2, a crucial component of the MAPK pathway, plays a vital role in regulating cellular responses to external stimuli, such as growth factors and stress signals. Dysregulation of the MAPK pathway, including aberrant MEK2 activity, has been implicated in various diseases, including cancer and developmental disorders.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.

Product Name:[KO Validated] MEK2 Monoclonal Antibody
SKU:CAB19078
Size:20μL, 100μL
Reactivity:Human, Mouse
Clone Number:ARC0361
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:MLAR RKPV LPAL TINP TIAE GPSP TSEG ASEA NLVD LQKK LEEL ELDE QQKK RLEA FLTQ KAKV GELK DDDF ERIS ELGA GNGG VVTK VQHR PSGL IMAR
Tested Applications:WB ELISA
Recommended Dilution:
WB1:500 - 1:2000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:CFC4, MEK2, MKK2, MAPKK2, PRKMK2, [KO/KD Validated] MEK2
Positive Sample:Jurkat, Mouse kidney, Mouse lung, HeLa
Cellular Localization:Cytoplasm, Membrane, Peripheral Membrane Protein.
Calculated MW:44kDa
Observed MW:44kDa

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, cognitive disability, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene.

Purification MethodAffinity purification
Gene ID5605
RRIDAB_2862570
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from wild type (WT) and MEK2 knockout (KO) HeLa cells, using [KO Validated] MEK2 Rabbit mAb (CAB19078) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Product ImageWestern blot analysis of various lysates using [KO Validated] MEK2 Rabbit mAb (CAB19078) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
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