KO Validated Antibodies 2

Anti-MEK2 Antibody [KO Validated] (CAB19078)

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SKU:
CAB19078
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-MEK2 Antibody [KO Validated]
Antibody SKU:CAB19078
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:A synthesized peptide derived from human MEK2
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:Jurkat, Mouse kidney, Mouse lung, HeLa
Immunogen:A synthesized peptide derived from human MEK2
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:5605
Uniprot:P36507
Cellular Location:Cytoplasm, Membrane, Peripheral membrane protein
Calculated MW:44kDa
Observed MW:44kDa
Anti-MEK2 Antibody [KO Validated] (CAB19078)Western blot analysis of extracts from normal (control) and MEK2 knockout (KO) HeLa cells, using MEK2 antibody (CAB19078) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-MEK2 Antibody [KO Validated] (CAB19078)Western blot analysis of extracts of various cell lines, using MEK2 antibody (CAB19078) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Synonyms:CFC4, MAPKK2, MEK2, MKK2, PRKMK2, MAP2K2
Background:The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene. [provided by RefSeq, Jul 2008]
UniProt Protein Function:MEK2: a dual-specificity protein kinase of the STE7 kinase family. Phosphorylated and activated by Raf and Mos kinases. Phosphorylates a Thr and a Tyr residue in a Thr-Glu-Tyr sequence located in the activation loop of ERK2 and ERK3. A component of MAP kinase signal transduction pathways involved in mitogen growth factor signal transduction.
UniProt Protein Details:

Protein type:EC 2.7.12.2; Protein kinase, dual-specificity (non-receptor); Protein kinase, STE; Kinase, protein; STE group; STE7 family

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: Golgi apparatus; peroxisomal membrane; microtubule; internal side of plasma membrane; focal adhesion; mitochondrion; endoplasmic reticulum; early endosome; extracellular region; intercellular junction; cell cortex; cytosol; perinuclear region of cytoplasm; late endosome; cytoplasm; nucleus

Molecular Function:MAP kinase kinase activity; protein serine/threonine kinase activity; protein serine/threonine kinase activator activity; protein binding; protein-tyrosine kinase activity; protein serine/threonine/tyrosine kinase activity; protein complex scaffold; ATP binding; PDZ domain binding

Biological Process: epidermal growth factor receptor signaling pathway; axon guidance; fibroblast growth factor receptor signaling pathway; peptidyl-tyrosine phosphorylation; activation of MAPKK activity; nerve growth factor receptor signaling pathway; activation of MAPK activity; MyD88-independent toll-like receptor signaling pathway; MAPKKK cascade; stress-activated MAPK cascade; pathogenesis; toll-like receptor 3 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; regulation of stress-activated MAPK cascade; small GTPase mediated signal transduction; Ras protein signal transduction; insulin receptor signaling pathway; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; vascular endothelial growth factor receptor signaling pathway; toll-like receptor 4 signaling pathway

Disease: Cardiofaciocutaneous Syndrome 4

NCBI Summary:The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, cognitive disability, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:P36507
NCBI GenInfo Identifier:547915
NCBI Gene ID:5605
NCBI Accession:P36507.1
UniProt Related Accession:P36507
Molecular Weight:
NCBI Full Name:Dual specificity mitogen-activated protein kinase kinase 2
NCBI Synonym Full Names:mitogen-activated protein kinase kinase 2
NCBI Official Symbol:MAP2K2  
NCBI Official Synonym Symbols:CFC4; MEK2; MKK2; MAPKK2; PRKMK2  
NCBI Protein Information:dual specificity mitogen-activated protein kinase kinase 2
UniProt Protein Name:Dual specificity mitogen-activated protein kinase kinase 2
UniProt Synonym Protein Names:ERK activator kinase 2; MAPK/ERK kinase 2; MEK 2
UniProt Gene Name:MAP2K2  
UniProt Entry Name:MP2K2_HUMAN
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