Epigenetics & Nuclear Signaling Antibodies 1
Anti-MED22 Antibody (CAB13825)
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- SKU:
- CAB13825
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-MED22 Antibody |
| Antibody SKU: | CAB13825 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human MED22 (NP_852468.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, A-431, A-549, Mouse testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human MED22 (NP_852468.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAQQ RALP QSKE TLLQ SYNK RLKD DIKS IMDN FTEI IKTA KIED ETQV SRAT QGEQ DNYE MHVR AANI VRAG ESLM KLVS DLKQ FLIL NDFP SVNE AIDQ RNQQ LRTL QEEC DRKL ITLR DEIS IDLY ELEE EYYS SRYK |
| Gene ID: | 6837 |
| Uniprot: | Q15528 |
| Cellular Location: | Nucleus |
| Calculated MW: | 16kDa/22kDa |
| Observed MW: | 22kDa |
| Synonyms: | MED22, MED24, SURF5, surf-5, SRB6 |
| Background: | This gene encodes a protein component of the mediator complex, which functions in the regulation of transcription by bridging interactions between gene-specific regulatory factors, RNA polymerase II, and general transcription factors. Alternatively spliced transcript variants encoding different isoforms have been observed. |
| UniProt Protein Function: | SURF5: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 22 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 9q34.2 Cellular Component: cytoplasm; Srb-mediator complex Molecular Function:protein binding Biological Process: transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter |
| NCBI Summary: | This gene encodes a protein component of the mediator complex, which functions in the regulation of transcription by bridging interactions between gene-specific regulatory factors, RNA polymerase II, and general transcription factors. Alternatively spliced transcript variants encoding different isoforms have been observed. [provided by RefSeq, Jul 2013] |
| UniProt Code: | Q15528 |
| NCBI GenInfo Identifier: | 6175074 |
| NCBI Gene ID: | 6837 |
| NCBI Accession: | Q15528.2 |
| UniProt Secondary Accession: | Q15528,O76072, Q5T8U0, B3KW83, B3KWX4, |
| UniProt Related Accession: | Q15528 |
| Molecular Weight: | 16,480 Da |
| NCBI Full Name: | Mediator of RNA polymerase II transcription subunit 22 |
| NCBI Synonym Full Names: | mediator complex subunit 22 |
| NCBI Official Symbol: | MED22 |
| NCBI Official Synonym Symbols: | MED24; SURF5; surf-5 |
| NCBI Protein Information: | mediator of RNA polymerase II transcription subunit 22; surfeit locus protein 5 |
| UniProt Protein Name: | Mediator of RNA polymerase II transcription subunit 22 |
| UniProt Synonym Protein Names: | Mediator complex subunit 22; Surfeit locus protein 5; Surf-5 |
| Protein Family: | Mediator of RNA polymerase II transcription |
| UniProt Gene Name: | MED22 |
| UniProt Entry Name: | MED22_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MED22 antibody (CAB13825) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s. |
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