Antibody Name:	Anti-ME3 Antibody
Antibody SKU:	CAB15787
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 505-604 of human ME3 (NP_001014811.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HT-29, 293T, Mouse brain, Mouse heart, Mouse kidney, Rat brain, Rat heart, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 505-604 of human ME3 (NP_001014811.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	IRHI PDEI FLLT AEQI AQEV SEQH LSQG RLYP PLST IRDV SLRI AIKV LDYA YKHN LASY YPEP KDKE AFVR SLVY TPDY DSFT LDSY TWPK EAMN VQTV
Gene ID:	10873
Uniprot:	Q16798
Cellular Location:	Mitochondrion matrix
Calculated MW:	38kDa/67kDa
Observed MW:	78kDa

Synonyms:

ME3, NADP-ME

Background:

Malic enzyme catalyzes the oxidative decarboxylation of malate to pyruvate using either NAD+ or NADP+ as a cofactor. Mammalian tissues contain 3 distinct isoforms of malic enzyme: a cytosolic NADP(+)-dependent isoform, a mitochondrial NADP(+)-dependent isoform, and a mitochondrial NAD(+)-dependent isoform. This gene encodes a mitochondrial NADP(+)-dependent isoform. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.

UniProt Protein Function:	ME3: Malic enzyme catalyzes the oxidative decarboxylation of malate to pyruvate using either NAD+ or NADP+ as a cofactor. Mammalian tissues contain 3 distinct isoforms of malic enzyme: a cytosolic NADP(+)-dependent isoform, a mitochondrial NADP(+)-dependent isoform, and a mitochondrial NAD(+)-dependent isoform. This gene encodes a mitochondrial NADP(+)-dependent isoform. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]
UniProt Protein Details:	Protein type:Carbohydrate Metabolism - pyruvate; EC 1.1.1.40; Mitochondrial; Oxidoreductase Chromosomal Location of Human Ortholog: 11cen-q22.3 Cellular Component: mitochondrial matrix; mitochondrion Molecular Function:cofactor binding; malate dehydrogenase (decarboxylating) activity; malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity; malic enzyme activity; metal ion binding; NAD binding; oxaloacetate decarboxylase activity Biological Process: aerobic respiration; malate metabolic process; pyruvate metabolic process
NCBI Summary:	Malic enzyme catalyzes the oxidative decarboxylation of malate to pyruvate using either NAD+ or NADP+ as a cofactor. Mammalian tissues contain 3 distinct isoforms of malic enzyme: a cytosolic NADP(+)-dependent isoform, a mitochondrial NADP(+)-dependent isoform, and a mitochondrial NAD(+)-dependent isoform. This gene encodes a mitochondrial NADP(+)-dependent isoform. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]
UniProt Code:	Q16798
NCBI GenInfo Identifier:	215274021
NCBI Gene ID:	10873
NCBI Accession:	Q16798.2
UniProt Secondary Accession:	Q16798,Q8TBJ0, B7Z6V0,
UniProt Related Accession:	Q16798
Molecular Weight:	38,626 Da
NCBI Full Name:	NADP-dependent malic enzyme, mitochondrial
NCBI Synonym Full Names:	malic enzyme 3, NADP(+)-dependent, mitochondrial
NCBI Official Symbol:	ME3
NCBI Official Synonym Symbols:	NADP-ME
NCBI Protein Information:	NADP-dependent malic enzyme, mitochondrial
UniProt Protein Name:	NADP-dependent malic enzyme, mitochondrial
UniProt Synonym Protein Names:	Malic enzyme 3
UniProt Gene Name:	ME3
UniProt Entry Name:	MAON_HUMAN

	Western blot analysis of extracts of various cell lines, using ME3 antibody (CAB15787) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.
	Immunohistochemistry of paraffin-embedded Mouse heart using ME3 Rabbit pAb (CAB15787) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Rat ovary using ME3 Rabbit pAb (CAB15787) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Human colon carcinoma using ME3 Rabbit pAb (CAB15787) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of L929 cells using ME3 antibody (CAB15787) at dilution of 1:100. Blue: DAPI for nuclear staining.