Developmental Biology
Anti-MDK Antibody (CAB0251)
- SKU:
- CAB0251
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Description
| Antibody Name: | Anti-MDK Antibody |
| Antibody SKU: | CAB0251 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 21-143 of human MDK (NP_002382.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SW480 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 21-143 of human MDK (NP_002382.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VAKK KDKV KKGG PGSE CAEW AWGP CTPS SKDC GVGF REGT CGAQ TQRI RCRV PCNW KKEF GADC KYKF ENWG ACDG GTGT KVRQ GTLK KARY NAQC QETI RVTK PCTP KTKA KAKA KKGK GKD |
| Gene ID: | 4192 |
| Uniprot: | P21741 |
| Cellular Location: | Secreted |
| Calculated MW: | 9kDa/15kDa |
| Observed MW: | 17kDa |
| Synonyms: | MDK, ARAP, MK, NEGF2, midkine |
| Background: | This gene encodes a member of a small family of secreted growth factors that binds heparin and responds to retinoic acid. The encoded protein promotes cell growth, migration, and angiogenesis, in particular during tumorigenesis. This gene has been targeted as a therapeutic for a variety of different disorders. Alternatively spliced transcript variants encoding multiple isoforms have been observed. |
| UniProt Protein Function: | MDK: Developmentally regulated, secreted growth factor homologous to pleiotrophin (PTN), which has heparin binding activity. Binds anaplastic lymphoma kinase (ALK) which induces ALK activation and subsequent phosphorylation of the insulin receptor substrate (IRS1), followed by the activation of mitogen-activated protein kinase (MAPK) and PI3-kinase, and the induction of cell proliferation. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development. Belongs to the pleiotrophin family. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 11p11.2 Cellular Component: cell projection; cytoplasm; extracellular region Molecular Function:heparin binding; growth factor activity Biological Process: response to drug; nervous system development; cell migration; Notch signaling pathway; dentate gyrus development; behavioral fear response; cerebellar granular layer development; short-term memory; adrenal gland development; positive regulation of transcription, DNA-dependent; response to glucocorticoid stimulus; signal transduction; positive regulation of cell division; response to wounding; cerebral cortex development; negative regulation of neuron apoptosis; cell differentiation; defecation; regulation of behavior |
| NCBI Summary: | This gene encodes a member of a small family of secreted growth factors that binds heparin and responds to retinoic acid. The encoded protein promotes cell growth, migration, and angiogenesis, in particular during tumorigenesis. This gene has been targeted as a therapeutic for a variety of different disorders. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2012] |
| UniProt Code: | P21741 |
| NCBI GenInfo Identifier: | 127116 |
| NCBI Gene ID: | 4192 |
| NCBI Accession: | P21741.1 |
| UniProt Secondary Accession: | P21741,Q2LEK4, Q9UCC7, |
| UniProt Related Accession: | P21741 |
| Molecular Weight: | 9,512 Da |
| NCBI Full Name: | Midkine |
| NCBI Synonym Full Names: | midkine (neurite growth-promoting factor 2) |
| NCBI Official Symbol: | MDK |
| NCBI Official Synonym Symbols: | MK; ARAP; NEGF2 |
| NCBI Protein Information: | midkine; retinoic acid inducible factor; amphiregulin-associated protein; midgestation and kidney protein; neurite outgrowth-promoting factor 2 |
| UniProt Protein Name: | Midkine |
| UniProt Synonym Protein Names: | Amphiregulin-associated protein; ARAP; Midgestation and kidney protein; Neurite outgrowth-promoting factor 2; Neurite outgrowth-promoting protein |
| Protein Family: | Midkine |
| UniProt Gene Name: | MDK |
| UniProt Entry Name: | MK_HUMAN |
 | Western blot analysis of extracts of SW480 cells, using MDK antibody (CAB0251). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
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