Developmental Biology
Anti-MDFI Antibody (CAB13709)
- SKU:
- CAB13709
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Description
| Antibody Name: | Anti-MDFI Antibody |
| Antibody SKU: | CAB13709 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human MDFI (NP_005577.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse heart, Rat heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human MDFI (NP_005577.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MYQV SGQR PSGC DAPY GAPS AAPG PAQT LSLL PGLE VVTG STHP AEAA PEEG SLEE AATP MPQG NGPG IPQG LDST DLDV PTEA VTCQ PQGN PLGC TPLL PNDS GHPS ELGG TRRA GNGA LGGP KAHR KLQT HPSL ASQG SKKS KSSS KSTT SQIP LQAQ |
| Gene ID: | 4188 |
| Uniprot: | Q99750 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 25kDa |
| Observed MW: | 35kDa |
| Synonyms: | MDFI, I-MF, I-mfa |
| Background: | This protein is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures. |
| UniProt Protein Function: | Inhibits the transactivation activity of the Myod family of myogenic factors and represses myogenesis. Acts by associating with Myod family members and retaining them in the cytoplasm by masking their nuclear localization signals. Can also interfere with the DNA-binding activity of Myod family members. Plays an important role in trophoblast and chondrogenic differentiation. Regulates the transcriptional activity of TCF7L1/TCF3 by interacting directly with TCF7L1/TCF3 and preventing it from binding DNA. Binds to the axin complex, resulting in an increase in the level of free beta-catenin. Affects axin regulation of the WNT and JNK signaling pathways. |
| NCBI Summary: | This protein is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q99750 |
| NCBI GenInfo Identifier: | 663853311 |
| NCBI Gene ID: | 4188 |
| NCBI Accession: | NP_001287733.1 |
| UniProt Related Accession: | Q99750 |
| Molecular Weight: | 25 KD |
| NCBI Full Name: | myoD family inhibitor isoform 1 |
| NCBI Synonym Full Names: | MyoD family inhibitor |
| NCBI Official Symbol: | MDFI |
| NCBI Official Synonym Symbols: | I-MF; I-mfa |
| NCBI Protein Information: | myoD family inhibitor |
| UniProt Protein Name: | MyoD family inhibitor |
| UniProt Synonym Protein Names: | Myogenic repressor I-mf |
| Protein Family: | MyoD family inhibitor |
| UniProt Gene Name: | MDFI |
 | Western blot analysis of extracts of various cell lines, using MDFI antibody (CAB13709) at 1:3000 dilution. |
 | Immunofluorescence analysis of U-2 OS cells using MDFI Rabbit pAb (CAB13709) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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