Cell Cycle Antibodies 1

Anti-MASTL Antibody (CAB14294)

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SKU:
CAB14294
Product Type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-MASTL Antibody
Antibody SKU:CAB14294
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 580-879 of human MASTL (NP_001165774.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse, Rat
Positive Samples:Mouse kidney, Mouse liver, Rat testis, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 580-879 of human MASTL (NP_001165774.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ESPL ESQP LDSD RSIK ESSF EESN IEDP LIVT PDCQ EKTS PKGV ENPA VQES NQKM LGPP LEVL KTLA SKRN AVAF RSFN SHIN ASNN SEPS RMNM TSLD AMDI SCAY SGSY PMAI TPTQ KRRS CMPH QQTP NQIK SGTP YRTP KSVR RGVA PVDD GRIL GTPD YLAP ELLL GRAH GPAV DWWA LGVC LFEF LTGI PPFN DETP QQVF QNIL KRDI PWPE GEEK LSDN AQSA VEIL LTID DTKR AGMK ELKR HPLF SDVD WENL QHQT MPFI PQPD DETD TSYF EARN TAQH LTVS GFSL
Gene ID:84930
Uniprot:Q96GX5
Cellular Location:Cleavage furrow, Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center
Calculated MW:92kDa/97kDa
Observed MW:110kDa
Synonyms:MASTL, GREATWALL, GW, GWL, MAST-L, THC2
Background:This gene encodes a microtubule-associated serine/threonine kinase. Mutations at this locus have been associated with autosomal dominant thrombocytopenia, also known as thrombocytopenia-2. Alternatively spliced transcript variants have been described for this locus.
UniProt Protein Function:MASTL: Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited. Phosphorylates histone protein in vitro; however such activity is unsure in vivo. May be involved in megakaryocyte differentiation. Defects in MASTL are the cause of thrombocytopenia type 2 (THC2). Thrombocytopenia is defined by a decrease in the number of platelets in circulating blood, resulting in the potential for increased bleeding and decreased ability for clotting. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Protein kinase, AGC; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; AGC group; MAST family

Chromosomal Location of Human Ortholog: 10p12.1

Cellular Component: nucleoplasm; centrosome; cytoplasm; nucleus; cleavage furrow

Molecular Function:protein serine/threonine kinase activity; protein phosphatase 2A binding; kinase activity; ATP binding

Biological Process: mitosis; regulation of cell cycle; cell division; mitotic cell cycle; G2/M transition of mitotic cell cycle; negative regulation of protein phosphatase type 2A activity; protein amino acid phosphorylation; response to DNA damage stimulus

Disease: Thrombocytopenia 2

NCBI Summary:This gene encodes a microtubule-associated serine/threonine kinase. Mutations at this locus have been associated with autosomal dominant thrombocytopenia, also known as thrombocytopenia-2. Alternatively spliced transcript variants have been described for this locus. [provided by RefSeq, Feb 2010]
UniProt Code:Q96GX5
NCBI GenInfo Identifier:288806587
NCBI Gene ID:84930
NCBI Accession:NP_001165774.1
UniProt Secondary Accession:Q96GX5,Q5T8D5, Q5T8D7, Q8NCD6, Q96SJ5,
UniProt Related Accession:Q96GX5
Molecular Weight:97,319 Da
NCBI Full Name:serine/threonine-protein kinase greatwall isoform 1
NCBI Synonym Full Names:microtubule associated serine/threonine kinase-like
NCBI Official Symbol:MASTL  
NCBI Official Synonym Symbols:GW; GWL; THC2; hGWL; MAST-L; GREATWALL; RP11-85G18.2  
NCBI Protein Information:serine/threonine-protein kinase greatwall; greatwall protein kinase; microtubule-associated serine/threonine-protein kinase-like
UniProt Protein Name:Serine/threonine-protein kinase greatwall
UniProt Synonym Protein Names:Microtubule-associated serine/threonine-protein kinase-like
UniProt Gene Name:MASTL  
UniProt Entry Name:GWL_HUMAN
Anti-MASTL Antibody (CAB14294)Western blot analysis of extracts of various cell lines, using MASTL antibody (CAB14294) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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