Developmental Biology

Anti-MARK2 Antibody (CAB16706)

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SKU:
CAB16706
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Developmental Biology

Description

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Antibody Name:Anti-MARK2 Antibody
Antibody SKU:CAB16706
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human MARK2
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, Raji, BxPC-3, BT-474, mouse testis, mouse heart, rat testis
Immunogen:Recombinant protein of human MARK2
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:2011
Uniprot:Q7KZI7
Cellular Location:Cell membrane, Cytoplasm, Lateral cell membrane, Peripheral membrane protein, cytoskeleton
Calculated MW:77-87kDa
Observed MW:78kDa
Synonyms:MARK2, EMK-1, EMK1, PAR-1, Par-1b, Par1b
Background:This gene encodes a member of the Par-1 family of serine/threonine protein kinases. The protein is an important regulator of cell polarity in epithelial and neuronal cells, and also controls the stability of microtubules through phosphorylation and inactivation of several microtubule-associating proteins. The protein localizes to cell membranes. Multiple transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:MARK2: a CAMK protein kinase of the CAMKL group. Plays a role in epithelial morphogenesis. Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells. One or more isoforms may play a role in graft rejection. 12 isoforms of the human protein are produced by alternative promoter.
UniProt Protein Details:

Protein type:Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; EC 2.7.11.26; CAMK group; CAMKL family; MARK subfamily

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: membrane; mitochondrion; plasma membrane; actin filament; basal cortex; nucleus; lateral plasma membrane

Molecular Function:protein serine/threonine kinase activity; protein binding; tau-protein kinase activity; magnesium ion binding; protein kinase activator activity; lipid binding; ATP binding

Biological Process: establishment and/or maintenance of epithelial cell polarity; Wnt receptor signaling pathway; establishment of cell polarity; protein amino acid autophosphorylation; peptidyl-threonine phosphorylation; mitochondrion degradation; neuron migration; activation of protein kinase activity; mitochondrion localization; regulation of axonogenesis; protein amino acid phosphorylation; regulation of cytoskeleton organization and biogenesis

NCBI Summary:This gene encodes a member of the Par-1 family of serine/threonine protein kinases. The protein is an important regulator of cell polarity in epithelial and neuronal cells, and also controls the stability of microtubules through phosphorylation and inactivation of several microtubule-associating proteins. The protein localizes to cell membranes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2009]
UniProt Code:Q7KZI7
NCBI GenInfo Identifier:62510922
NCBI Gene ID:2011
NCBI Accession:Q7KZI7.2
UniProt Secondary Accession:Q7KZI7,Q15449, Q15524, Q5XGA3, Q68A18, Q96HB3, Q96RG0
UniProt Related Accession:Q7KZI7
Molecular Weight:788
NCBI Full Name:Serine/threonine-protein kinase MARK2
NCBI Synonym Full Names:MAP/microtubule affinity-regulating kinase 2
NCBI Official Symbol:MARK2  
NCBI Official Synonym Symbols:EMK1; EMK-1; PAR-1; Par1b; Par-1b  
NCBI Protein Information:serine/threonine-protein kinase MARK2; PAR1 homolog b; ELKL motif kinase 1; Ser/Thr protein kinase PAR-1B; serine/threonine protein kinase EMK
UniProt Protein Name:Serine/threonine-protein kinase MARK2
UniProt Synonym Protein Names:ELKL motif kinase 1; EMK-1; MAP/microtubule affinity-regulating kinase 2; PAR1 homolog; PAR1 homolog b; Par-1b; Par1b
Protein Family:Serine/threonine-protein kinase
UniProt Gene Name:MARK2  
UniProt Entry Name:MARK2_HUMAN
Anti-MARK2 Antibody (CAB16706)Western blot analysis of extracts of various cell lines, using MARK2 antibody (CAB16706) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-MARK2 Antibody (CAB16706)Immunofluorescence analysis of L929 cells using MARK2 antibody (CAB16706) at dilution of 1:100. Blue: DAPI for nuclear staining.
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