KO Validated Antibodies 1

Anti-MAPK3 Antibody (CAB0228)[KO Validated]

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SKU:
CAB0228
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death

Description

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Antibody Name:Anti-MAPK3 Antibody [KO Validated]
Antibody SKU:CAB0228
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MAPK3 (NP_002737.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:DU145, HeLa, 293T
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MAPK3 (NP_002737.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAAA AAQG GGGG EPRR TEGV GPGV PGEV EMVK GQPF DVGP RYTQ LQYI GEGA YGMV SSAY DHVR KTRV AIKK ISPF EHQT YCQR TLRE IQIL LRFR HENV
Gene ID:5595
Uniprot:P27361
Cellular Location:Cytoplasm, Nucleus
Calculated MW:38kDa/40kDa/43kDa
Observed MW:40kDa
Synonyms:ERK-1, ERK1, ERT2, HS44KDAP, HUMKER1A, P44ERK1, P44MAPK, PRKM3, p44-ERK1, p44-MAPK, MAPK3
Background:The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described.
UniProt Protein Function:ERK1: a serine/threonine kinase of the GMGC group that plays a critical role in the regulation of cell growth and differentiation. ERK1 (MAPK3) and ERK2 (MAPK1) play central roles in MAPK cascades and are activated by a wide variety of extracellular signals including growth and neurotrophic factors, cytokines, hormones and neurotransmitters. Depending on the cellular context, MAPK cascades mediate diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. MAPK cascades also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. Activation of MAP kinases occurs through phosphorylation of threonine and tyrosine residues at the sequence T*EY* by upstream MAP kinase kinases, MEK1 and -2. Phosphorylation of both the threonine and tyrosine are required for activity. This phosphorylation causes dramatic conformational changes, which enable full activation and interaction of MAPK1/ERK2 with its substrates.
UniProt Protein Details:

Protein type:EC 2.7.11.24; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; CMGC group; MAPK family; ERK subfamily; MAPK/ERK subfamily

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: microtubule cytoskeleton; nucleoplasm; Golgi apparatus; focal adhesion; cytoskeleton; mitochondrion; late endosome; early endosome; nuclear envelope; caveola; pseudopodium; nucleus; cytosol

Molecular Function:MAP kinase activity; protein binding; phosphotyrosine binding; ATP binding; phosphatase binding

Biological Process: axon guidance; DNA damage induced protein phosphorylation; activation of MAPKK activity; nerve growth factor receptor signaling pathway; positive regulation of histone phosphorylation; viral reproduction; apoptosis; activation of MAPK activity; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; sensory perception of pain; protein amino acid phosphorylation; toll-like receptor 10 signaling pathway; BMP signaling pathway; toll-like receptor 5 signaling pathway; response to exogenous dsRNA; regulation of transcription factor activity; small GTPase mediated signal transduction; lipopolysaccharide-mediated signaling pathway; toll-like receptor 4 signaling pathway; epidermal growth factor receptor signaling pathway; platelet activation; fibroblast growth factor receptor signaling pathway; cytokine and chemokine mediated signaling pathway; MyD88-independent toll-like receptor signaling pathway; MAPKKK cascade; transcription from RNA polymerase I promoter; cell cycle; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; regulation of stress-activated MAPK cascade; organ morphogenesis; cartilage development; Ras protein signal transduction; toll-like receptor signaling pathway; insulin receptor signaling pathway; innate immune response; gene expression; positive regulation of histone acetylation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; toll-like receptor 9 signaling pathway; transcription initiation from RNA polymerase I promoter; vascular endothelial growth factor receptor signaling pathway; blood coagulation; phosphorylation; regulation of cytoskeleton organization and biogenesis

NCBI Summary:The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008]
UniProt Code:P27361
NCBI GenInfo Identifier:232066
NCBI Gene ID:5595
NCBI Accession:P27361.4
UniProt Secondary Accession:P27361,Q8NHX1, A8CZ58, B0LPG3,
UniProt Related Accession:P27361
Molecular Weight:379
NCBI Full Name:Mitogen-activated protein kinase 3
NCBI Synonym Full Names:mitogen-activated protein kinase 3
NCBI Official Symbol:MAPK3
NCBI Official Synonym Symbols:ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
NCBI Protein Information:mitogen-activated protein kinase 3; MAPK 1; MAP kinase isoform p44; insulin-stimulated MAP2 kinase; extracellular signal-related kinase 1; extracellular signal-regulated kinase 1; microtubule-associated protein 2 kinase
UniProt Protein Name:Mitogen-activated protein kinase 3
UniProt Synonym Protein Names:ERT2; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; p44-MAPK; Microtubule-associated protein 2 kinase; p44-ERK1
UniProt Gene Name:MAPK3
UniProt Entry Name:MK03_HUMAN
Anti-MAPK3 Antibody [KO Validated] (CAB0228)Western blot analysis of extracts of various cell lines, using MAPK3 antibody (CAB0228) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-MAPK3 Antibody [KO Validated] (CAB0228)Western blot analysis of extracts from normal (control) and MAPK3 Rabbit pAb knockout (KO) 293T cells, using MAPK3 Rabbit pAb antibody (CAB0228) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-MAPK3 Antibody [KO Validated] (CAB0228)Immunohistochemistry of paraffin-embedded rat lung using MAPK3 Antibody (CAB0228) at dilution of 1:100 (40x lens).
Anti-MAPK3 Antibody [KO Validated] (CAB0228)Immunohistochemistry of paraffin-embedded human kidney using MAPK3 Antibody (CAB0228) at dilution of 1:100 (40x lens).
Anti-MAPK3 Antibody [KO Validated] (CAB0228)Immunohistochemistry of paraffin-embedded mouse heart using MAPK3 Antibody (CAB0228) at dilution of 1:100 (40x lens).
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