KO Validated Antibodies 1

Anti-MAP2K4 Antibody (CAB14781)[KO Validated]

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SKU:
CAB14781
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death

Description

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Antibody Name:Anti-MAP2K4 Antibody [KO Validated]
Antibody SKU:CAB14781
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 300-399 of human MAP2K4 (NP_003001.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:SH-SY5Y, BT-474, MCF-7, mouse thymus, mouse liver, rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 300-399 of human MAP2K4 (NP_003001.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LATG RFPY PKWN SVFD QLTQ VVKG DPPQ LSNS EERE FSPS FINF VNLC LTKD ESKR PKYK ELLK HPFI LMYE ERAV EVAC YVCK ILDQ MPAT PSSP MYVD
Gene ID:6416
Uniprot:P45985
Cellular Location:Cytoplasm, Nucleus
Calculated MW:44kDa/45kDa
Observed MW:44kDa
Synonyms:MAP2K4, JNKK, JNKK1, MAPKK4, MEK4, MKK4, PRKMK4, SAPKK-1, SAPKK1, SEK1, SERK1, SKK1
Background:This gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:MKK4: dual specificity kinase of the STE7 family that phosphorylates and activates JNK1 and -2 as well as p38 but not ERK1 or -2. Mediates cellular responses to various cellular stresses and inflammatory cytokines. Phosphorylation by Akt inhibits MKK4 and suppresses stress-activated signal transduction.
UniProt Protein Details:

Protein type:Protein kinase, STE; Protein kinase, dual-specificity (non-receptor); Kinase, protein; EC 2.7.12.2; STE group; STE7 family

Chromosomal Location of Human Ortholog: 17p12

Cellular Component: dendrite cytoplasm; axon; perikaryon; intracellular; cytosol; nucleus

Molecular Function:protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; mitogen-activated protein kinase kinase kinase binding; JUN kinase kinase activity; ATP binding; protein kinase activity

Biological Process: peptidyl-tyrosine phosphorylation; apoptosis; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; pathogenesis; toll-like receptor 3 signaling pathway; signal transduction; activation of JNK activity; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; positive regulation of neuron apoptosis; toll-like receptor signaling pathway; JNK cascade; innate immune response; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway; positive regulation of DNA replication; positive regulation of nitric-oxide synthase biosynthetic process

NCBI Summary:This gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
UniProt Code:P45985
NCBI GenInfo Identifier:1170596
NCBI Gene ID:6416
NCBI Accession:P45985.1
UniProt Secondary Accession:P45985,Q5U0B8, Q6FHX4, Q6P9H2, Q6PIE6, B2R7N7, B3KYB2 D3DTS5,
UniProt Related Accession:P45985
Molecular Weight:399
NCBI Full Name:Dual specificity mitogen-activated protein kinase kinase 4
NCBI Synonym Full Names:mitogen-activated protein kinase kinase 4
NCBI Official Symbol:MAP2K4  
NCBI Official Synonym Symbols:JNKK; MEK4; MKK4; SEK1; SKK1; JNKK1; SERK1; MAPKK4; PRKMK4; SAPKK1; SAPKK-1  
NCBI Protein Information:dual specificity mitogen-activated protein kinase kinase 4; MEK 4; MAPKK 4; MAPK/ERK kinase 4; SAPK/ERK kinase 1; MAP kinase kinase 4; JNK-activated kinase 1; JNK-activating kinase 1; c-Jun N-terminal kinase kinase 1; stress-activated protein kinase kinase 1
UniProt Protein Name:Dual specificity mitogen-activated protein kinase kinase 4
UniProt Synonym Protein Names:JNK-activating kinase 1; MAPK/ERK kinase 4; MEK 4; SAPK/ERK kinase 1; SEK1; Stress-activated protein kinase kinase 1; SAPK kinase 1; SAPKK-1; SAPKK1; c-Jun N-terminal kinase kinase 1; JNKK
Protein Family:Dual specificity mitogen-activated protein kinase kinase
UniProt Gene Name:MAP2K4  
UniProt Entry Name:MP2K4_HUMAN
Anti-MAP2K4 Antibody [KO Validated] (CAB14781)Western blot analysis of extracts of various cell lines, using MAP2K4 antibody (CAB14781) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-MAP2K4 Antibody [KO Validated] (CAB14781)Western blot analysis of extracts from normal (control) and MAP2K4 knockout (KO) HeLa cells, using MAP2K4 antibody (CAB14781) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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